Cel The objective are: - to develop a new type of vaccine for African horsesickness (AHS) disease; - to develop a diagnostic reagent to differentiate between vaccinated and infected animals. Specific objectives are: - To express 2 or 4 capsid proteins of AHSV-4 using available cDNA clones and baculovirus vectors and synthesize empty virus-like particles (VLPs). - To evaluate the immunological properties of VLPs in model animals (e.g. mice). - To characterize humoral and cell-mediated immunity elicited by VLPs. - To evaluate protective efficiency of VLPs in natural vertebrate hosts (e.g. horse). - To synthesize a non-structural viral-coded AHSV NS1 protein (ie a non-capsid protein) using a baculovirus vector and to caharacterise the expressed protein immunogically and biochemically. - To purify the expressed protein and use the protein to distinguish between vaccinated and naturally-infected animals. - To generate cDNA clones of other AHSV serotypes that are needed to synthesize VLPs for other serotypes. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccines Program(-y) FP3-AIR - Specific research and technological development and demonstration programme (EEC) in the field of agriculture and agro-industry, including fisheries, 1990-1994 Temat(-y) 232 - Animal health and well being Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator University of Oxford Wkład UE Brak danych Adres South Parks Road OX1 3QU Oxford Zjednoczone Królestwo Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (1) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko ASSOCIACAO PARA DESENVOLVIMENTO CIENCIAS VETERINARIAS Portugalia Wkład UE Brak danych Adres APARTADO 12182 1058 LISBOA Zobacz na mapie Koszt całkowity Brak danych