Cel The aim of this proposal is to carry out a systematic search for the function of all members of one family of transcription factors in Arabidopsis. The members of the myb gene family contain a highly conserved DNA-binding motif, which has permitted the isolation of partial cDNAs representing over 80 members of the Arabidopsis myb gene family as part of the Biotech and Arabidopsis genome programmes. The functions of a few of these genes are beginning to be understood using a combination of genetical and biochemical analysis in several species. Consensus target sites in promoters have been identified, and some Myb proteins have been shown to activate transcription of target genes encoding enzymes of biochemical pathways. Other members of the family control cellular differentiation, and plant responses to environmental challenges such as UV light, water stress, mechanical damage and defence responses against pathogens. However, it is clear that because the functions of most of the members of this unusually large gene family are unknown, and because of the important and diverse cellular functions of characterised Mybs, the full range of the regulatory influence of this gene family is not yet appreciated and promises to be very significant. This project aims to identify the function of most members the myb gene family in Arabidopsis using a PCR-based strategy to screen systematically for insertion mutations in each of the myb genes. Such strategies have been successfully established in Drosophila and Caenorhabditis, and have recently been applied to mutagenized plant populations. Such a systematic search promises to be both the most efficient route for determining gene function and will also provide the most comprehensive and readily applicable knowledge of the genes and the interactions between members of the family. Publicly available sources of insertion mutations include the 14,000 T-DNA tagged lines of Feldmann and coworkers, which harbour insertions in more than 65% of Arabidopsis genes, and an allied function search proposal aiming to use T-DNA and transposons to saturate the genome with insertions. As a means of detecting mutations in genes of unknown function, insertional mutagenesis is considerably more efficient in plants than gene inactivation through homologous recombination. Despite intensive efforts to design methods for observing gene replacement in plants, the phenomenon remains very poorly characterised and as a practical method will not provide any opportunity for use at a time when systematic function searches promise to be so fruitful, as accompanying measures to ensure the maximal use of genome sequencing programmes. The planned work will also establish a model for interactions between EU labs which exploit the resources available from the genome programme, the programme(s) aimed at saturation mutagenesis of the Arabidopsis genome, and those labs with common biological interests and goals. Such a combination of resources, achievable only in a pan-European context, promises to lay new foundations for efficient and effective studies in fundamental aspects of plant biology which can be directly applied to agricultural improvement. Dziedzina nauki natural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Program(-y) FP4-BIOTECH 2 - Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998 Temat(-y) 020204 - New methods for genome analysis Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator AMICA SCIENCE EEIG Wkład UE Brak danych Adres Norwich Research Park John Innes Centre, Colney NR4 7UH NORWICH Zjednoczone Królestwo Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (5) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Hiszpania Wkład UE Brak danych Adres 115,Campus Universidad Autonoma 28049 MADRID Zobacz na mapie Koszt całkowity Brak danych John Innes Centre Zjednoczone Królestwo Wkład UE Brak danych Adres Sainsbury Laboratory Colney Lane NR4 7UH Norwich Zobacz na mapie Koszt całkowity Brak danych Max-Planck-Gesellschaft zur Forderungder Wissenschaften e.V. Niemcy Wkład UE Brak danych Adres 10,Carl-von-Linné-Weg 50829 Köln Zobacz na mapie Koszt całkowity Brak danych THE UNIVERSITY OF MILANO Włochy Wkład UE Brak danych Adres Via Celoria 26 20133 MILANO Zobacz na mapie Koszt całkowity Brak danych UTRECHT UNIVERSITY Niderlandy Wkład UE Brak danych Adres 8,Padualaan 8 3584 CH UTRECHT Zobacz na mapie Koszt całkowity Brak danych
