Objetivo Traditional scale-up methods for aerobic microbial processes suffer from lack of precision, which makes industrial scale-up of biotechnical production unpredictable, slow and costly. Today, this problem is accentuated since so many new biotechnology based products are to be brought to the market and the speed to the market is a key to success. This project aims at developing an improved strategy for scale-up, that will be based on recent advances in biological and engineering sciences studying physiological stress responses, metabolism, measurement techniques and computational methods. This will be achieved by investigation of the interaction between the physical/chemical environment in different parts of the bioreactor, and the biological responses to the strongly scale dependent variations of this environment. Computer simulation programmes will be developed based on these results and it will be used for mapping of the bioreactor micro-environment and calculation of the biological reaction rates in these micro-environments. The sum of these reactions constitutes the performance of the bioreactor. Therefore the process results can be simulated under various scale-up conditions. Parameters that are specifically focused on for optimization of the productivity and product quality are formation of inhibitory by-products, physiological stress and foaming. The validity of the models and the strategy will be investigated by analyses of the reactor environment and the process performance in industrial scale-up experiments for production of an amino acid and a recombinant protein in industrial bioreactors at a scale of 30 and 10 cubic metres, respectively. The joint expertise of the group of laboratories and industries that will work to achieve the goal includes, molecular biology, physiology, chemical and physical analysis, fluid dynamics, mathematical modelling, chemical engineering, and industrial production of amino acids for animal feed, recombinant pharmaceutical proteins and biotransformation of fine chemicals. The project will generate i) methodologies and equipment to investigate scale-up sensitivity of microbial strains and, ii) improved methods to predict the industrial scale performance. Taken together, these results will generate methods to identify and quantify the need for improvement of i) the strain, ii) the bioreactor and/or iii) the process control, for a more predictable and efficient process scale-up. Ámbito científico engineering and technologyenvironmental biotechnologybioremediationbioreactorsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesclassical mechanicsfluid mechanicsfluid dynamicsnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesmathematicsapplied mathematicsmathematical model Programa(s) FP4-BIOTECH 2 - Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998 Tema(s) 0102 - Biochemical engineering components of cell factories Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador ROYAL INSTITUTE OF TECHNOLOGY Aportación de la UE Sin datos Dirección 34,Roslagstullsbacker, 21 100 44 STOCKHOLM Suecia Ver en el mapa Coste total Sin datos Participantes (12) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo DELFT UNIVERSITY OF TECHNOLOGY Países Bajos Aportación de la UE Sin datos Dirección Julianalaan 67 2628 BC DELFT Ver en el mapa Coste total Sin datos Ernst-Moritz-Arndt-Universität Greifswald Alemania Aportación de la UE Sin datos Dirección 15,Friedrich-Ludwig-Jahn-Strasse 17487 Greifswald Ver en el mapa Coste total Sin datos LUND UNIVERSITY Suecia Aportación de la UE Sin datos Dirección 60, 221 00 LUND Ver en el mapa Coste total Sin datos Lonza AG Suiza Aportación de la UE Sin datos Dirección 3930 Visp Ver en el mapa Coste total Sin datos Martin-Luther-Universitat Halle-Wittenberg Alemania Aportación de la UE Sin datos Dirección 16 A,Weinbergweg 16 A 6120 Halle Ver en el mapa Coste total Sin datos Multi Ferm AB Suecia Aportación de la UE Sin datos Dirección 47,S.t. Lars Väg 220 09 Lund Ver en el mapa Coste total Sin datos PHARMACIA & UPJOHN AB Suecia Aportación de la UE Sin datos Dirección 37,Mariefredsvaegen 37 64541 STRAENGNAES Ver en el mapa Coste total Sin datos Scaba AB Suecia Aportación de la UE Sin datos Dirección 12,Enhagsvägen 183 02 Täby Ver en el mapa Coste total Sin datos Telemark Innovation Centre Noruega Aportación de la UE Sin datos Dirección Kjølnes Ring 3900 Porsgrunn Ver en el mapa Coste total Sin datos UNIVERSITY OF BIRMINGHAM Reino Unido Aportación de la UE Sin datos Dirección Edgbaston B15 2TT Birmingham Ver en el mapa Coste total Sin datos University College Dublin Irlanda Aportación de la UE Sin datos Dirección Belfield 4 Dublin Ver en el mapa Coste total Sin datos Universität Stuttgart Alemania Aportación de la UE Sin datos Dirección Allmandring 31 70569 Stuttgart Ver en el mapa Coste total Sin datos