Objetivo To analyze the mechanisms underlying the release of nucleotides from the CNS and target areas.To identify new P2-purinoceptor subtypes and to synthesize novel P2 receptor subtype ligands, expected to be specific agonists/antagonists.To investigate the cellular responses to nucleotides and the intracellular signalling cascades in select examples of neural or glial cells.To characterize the enzymes involved in the extracellular hydrolysis of ATP and ADP.To reveal the pathological implications of extracellular nucleotides, with particular emphasis on nociception. Nucleotides, a novel group of signalling substances in the central and peripheral nervous system will be studied. These include ATP, ADP, UTP or the diadenosine polyphosphates Ap3A, Ap4A, Ap5A, and Ap6A. The release of ATP from nerve endings will be characterized including its function via presynaptic receptors as an autocrine substance. The action of nucleotides on their receptors and the induction of intracellular signalling pathways will be studied in select examples such as astrocytes and the sensory hair cells of the inner ear. This includes the differentiation by selective analogues between the various nucleotide receptor subtypes including those for nucleoside 5'-triphosphates and the diadenosine polyphosphates. New P2-receptor subtypes will be identified and isolated. The involvement of ATP and possibly other nucleotides in pathological mechanisms is investigated using nociception as an example. The enzymes that effect the extracellular hydrolysis of nucleotides such as ATP and ADP are characterized in functional and molecular terms. Their sites of expression in the nervous system will be determined using immunocytochemistry. Analogues are developed that differentiate between nucleotide receptor subtypes and that selectively affect either receptors or ecto-nucleotidase. These substances will be the basis for the development of drugs to deal with diseases based on specific faults in the signalling cascade via extracellular nucleotides. Ámbito científico natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencescell biologycell signalingnatural sciencesbiological sciencesgeneticsnucleotidesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998 Tema(s) 3.1 - Pathophysiology and basic mechanisms Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador Johann-Wolfgang-Goethe Universität Frankfurt Aportación de la UE Sin datos Dirección 9,Marie-Curie-Strasse 60439 Frankfurt-am-Main Alemania Ver en el mapa Coste total Sin datos Participantes (6) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Albert-Ludwigs-Universität Freiburg Alemania Aportación de la UE Sin datos Dirección 5,Hermann-Herder-Strasse 79104 FREIBURG Ver en el mapa Coste total Sin datos Stockholms Universitet Suecia Aportación de la UE Sin datos Dirección 21 A,Stockholm University 106 91 STOCKHOLM Ver en el mapa Coste total Sin datos THE UNIVERSITY OF MILANO Italia Aportación de la UE Sin datos Dirección Via Balzaretti 9 20133 MILANO Ver en el mapa Coste total Sin datos Universidad Complutense de Madrid España Aportación de la UE Sin datos Dirección S/N,Universidad Complutense 2840 MADRID Ver en el mapa Coste total Sin datos Universidade de Lisboa Portugal Aportación de la UE Sin datos Dirección Avenida Prof. Egas Moniz 1600 Lisboa Ver en el mapa Coste total Sin datos University College London Reino Unido Aportación de la UE Sin datos Dirección 5,Rowland Hill Street NW3 2PF LONDON Ver en el mapa Coste total Sin datos