Objetivo The aim of the proposed project is to develop procedures for gene and cell therapy for application in the treatment of neurological diseases, in particular Parkinson's disease and epilepsy. Approaches will involve gene therapy strategies using both ex vivo and in vivo direct injection to induce production of neurotransmitter and neurotrophic molecules. The project includes all levels of analysis and stages of development from the production of viral vectors, genetic manipulation and encapsulation of cells, in vivo gene transfer, to the preclinical assessment of procedures in preparation for clinical trials with patients. The proposed research addresses two neurological diseases: Parkinson' s disease (PD) and epilepsy, affections chosen because their pathophysiologies suggest possible therapeutic agents: neurotransmitter, trophic factors or both. Both ex vivo and in vivo gene therapy strategies will be developed in five partially overlapping phases:Types of cells suitable for genetic manipulation and intracerebral transplantation will be identified, and the tools to transfer genes for neurotransmitter-synthesising enzymes and neurotrophic factors constructed.Suitable types of cells intracerebrawill be identified and the tools to transfer genes for neurotransmitter-synthesising enzymes and neurotrophic factors constructed.Encapsulation techniques for the transplantation of genetically engineered allo and xenogeneic cell lines will be tested.The functional properties of the transduced cells in vitro and of recombinant adenoviral constructions will be analysed in detail, and the functional efficacy of DOPA/dopamine and neurotrophic factor-producing cells in well characterised in vivo models assessed.Alternative gene constructs where the transgene is under the control of endogenous, cell-specific promoters containing suitable regulatory elements will be studied. The aim is to obtain improved regulation and stable long-term expression of the transgenes in vivo. This will allow function of the transduced cells to be evaluated over longer time periods and in complex functional tests .The various methods developed within the programme will be validated in small animal models and a primate model of PD. Ámbito científico medical and health sciencesbasic medicineneurologyepilepsymedical and health sciencesmedical biotechnologygenetic engineeringgene therapymedical and health sciencesbasic medicineneurologyparkinsonmedical and health sciencesclinical medicinetransplantationmedical and health sciencesmedical biotechnologycells technologies Programa(s) FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998 Tema(s) 3.2 - Nervous system damage and repair Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador Centre National de la Recherche Scientifique Aportación de la UE Sin datos Dirección 83,Boulevard de l'Hopital 75013 Paris Francia Ver en el mapa Coste total Sin datos Participantes (3) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo CENTRE HOSPITALIER UNIVERSITAIRE VAUDOIS Suiza Aportación de la UE Sin datos Dirección 3,Pavillon 4 1011 LAUSANNE Ver en el mapa Coste total Sin datos Commissariat à l'Energie Atomique Francia Aportación de la UE Sin datos Dirección 4,Place Général Leclerc 91401 Orsay Ver en el mapa Coste total Sin datos Lunds Universitet Suecia Aportación de la UE Sin datos Dirección 17,Sölvegatan 223 62 Lund Ver en el mapa Coste total Sin datos