Objective The goal of this project is to study the mechanisms underlying the modulation by second messengers of nicotinic acetylcholine receptor (nAChR)-activated ionic channels in muscle, in neurons of invertebrates (snail) and in vertebrates (sympathetic ganglion of the rat). A combination of the following techniques will be used: electrophysiology (patch-clamp, microelectrode, and intracellular dialysis recording); chemistry (synthesis of new nACh receptor-channel modulators); and fluorescence image analysis (confocal laser scanning microscopy). The following areas will be investigated: the comparative properties of whole-cell AChR-activated currents in mouse myotubes and in sympathetic ganglia neurons; modulation of nAChR by four protein kinase systems (cAMP- and cGMP-dependent phosphorylation; the protein kinase C- and tyrosine kinase-dependent phosphorylation); the modulation of ACh-activated currents in snail neurons by cytoskeleton; the pathways involved in intracellular Ca 2+ ([Ca 2+]i) elevation at AChR-activation and the modulation of AChR channels by [Ca 2+]i.This study will make it possible to elucidate mechanisms of nAChR channels modulation by intracellular phosphorylation and by the state of neuronal cytoskeleton. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Topic(s) 42 - Physical Chemical Biology Call for proposal Data not available Funding Scheme Data not available Coordinator Institut National de la Santé et de la Recherche Médicale EU contribution No data Address Bld. de Port Royal 123 75674 Paris 14 France See on map Total cost No data Participants (4) Sort alphabetically Sort by EU Contribution Expand all Collapse all Institute of Cell Biophysics Russia EU contribution No data Address 142292 Pushchino, Moscow Region See on map Total cost No data National Academy of Sciences of Ukraine Ukraine EU contribution No data Address 252024 Kiev See on map Total cost No data Russian Academy of Sciences Russia EU contribution No data Address 197376 St. Petersburg See on map Total cost No data Università degli Studi di Roma La Sapienza Italy EU contribution No data Address 00158 Roma See on map Total cost No data
