Objective At the present time the causes of spoilage in vacuum-packaged cold-smoked fish, are largely unknown. Specific spoilage organisms have not been identified, nor the biochemical or physical course(s) of degradation known. Similarly, there are no agreed measures of quality of cold-smoked fish nor means of estimating shelf-life. This Proposal seeks to understand the causes of deterioration, and to establish objective quality indices. On determining the mechanisms of loss of quality, it will be possible to develop rapid, simple, quantitative, microbiological and physicochemical means for their quality measurement in cold-smoked fish, leading to potential methods for estimating shelf-life.Cold-smoked fish are often contaminated with Listeria monocytogenes, an organism capable of causing serious, often fatal, disease in susceptible individuals. Although no cases of listeriosis have been attributed to consumption of cold-smoked fish, it is clearly necessary to minimise the risk to consumers from this hazard in food. This Proposal will investigate the frequency of occurrence of this organism in European cold-smoked fish and the biotypes present, and compare these with biotypes isolated from other foods and from cases of listeriosis by reference to work in other laboratories. The Proposal will establish the source(s) of the organism, and the route(s) of contamination within processing facilities, by determining the spread of biotypes. The efficacy of fish processing and sanitising procedures in controlling Listeria spp. within the processing plants will be studied, and the effects of modifications to procedures evaluated.Several strains of inhibitory lactic acid bacteria are available from current or previous research projects funded by the EU Commission, and are held by some of the Partners in this Proposal. Such strains will be examined for their ability to kill or inhibit L. monocytogenes and specific spoilage organisms in conditions simulating cold-smoked fish. The application of these selected lactic acid bacteria and/or their inhibitory products (e.g. bacteriocins) and already shown to be effective against Listeria spp. and spoilage organisms in other situations, will be investigated as additional hurdles for the control or elimination of L. monocytogenes in cold-smoked fish, and to extend the shelf-life. Fields of science natural scienceschemical sciencesorganic chemistryorganic acidsnatural sciencesbiological sciencesmicrobiologybacteriology Programme(s) FP4-FAIR - Specific research, technological development and demonstration programme in the field of agriculture and fisheries (including agro-industry, food technologies, forestry, aquaculture and rural development), 1994-1998 Topic(s) 3.3.3 - Quality monitoring and quality assurance for fish and fish products Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator UNIVERSIDADE CATOLICA PORTUGUESA EU contribution No data Address Rua Dr Antonio Bernardino de Almeida 4200 PORTO Portugal See on map Total cost No data Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all Association sur l'Asepsie de Production France EU contribution No data Address Rue des Docteurs Calmette et Guerin 53020 Laval See on map Total cost No data Danish Institute for Fisheries Research Denmark EU contribution No data Address Soltofts Plads, c/o Technical University of Denmark, Bygning 221 2800 Lyngby See on map Total cost No data Ecole Nationale d'Ingenieurs des Techniques France EU contribution No data Address Domaine de la Geraudiere 44072 Nantes See on map Total cost No data IFREMER - DRV/VP France EU contribution No data Address Rue de l'Ile d'Yeu 44311 Nantes See on map Total cost No data Icelandic Fisheries Laboratories Iceland EU contribution No data Address Skulagata 4 121 Reykjavik See on map Total cost No data NETHERLANDS INSTITUTE FOR FISHERIES RESEARCH Netherlands EU contribution No data Address Haringkade 1 IJMUIDEN See on map Total cost No data The British Food Manufacturing Industries Research Association United Kingdom EU contribution No data Address Randalls Road KT22 7RY Leatherhead, Surrey See on map Total cost No data University College Cork Ireland EU contribution No data Address Western Road Cork See on map Total cost No data