Objectif This project will study basic inmunopathological mechanisms, including cellular and cytokine profiles, related to intraperitoneal immune granulomas seen in vaccinated farmed fish Atlantic salmon, rainbow trout and sea bass following the use of oil-adjuvant vaccines containing Aromas salmonicida, Vibrato angular or Pasteur Ella viscid. Emphasis on the contribution of eosinophilic granular cells/mast cells and of pro-inflammatory mediators and cytokines to granule formation. Techniques ranging from histocytoschemistry to gene expressions studies and ex vivo tissue explants will be used. Results contributing to the development of safer vaccine formulations for fish are expected.The project has shed light on the underlying mechanisms of granuloma formation in vaccinated farmed fish and it has been documented that the characteristics of the vaccine formulation, e.g. extracellular products, antigen content and stability has a bearing on the extent of injection site reactions. It has been shown that different cytokines are produced at the site of injection and that it is also up-regulated in immunocompetent organs like the spleen a few days after vaccination. Champ scientifique medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccines Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Thème(s) 1.1.1.-2. - Key action Control of Infectious Diseases Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur NORWEGIAN SCHOOL OF VETERINARY SCIENCE Contribution de l’UE Aucune donnée Adresse Ullevaalsveien 72 0033 OSLO Norvège Voir sur la carte Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire ALPHARMA AS Norvège Contribution de l’UE Aucune donnée Adresse Harbitzalleen 3 Skoyen 0212 OSLO Voir sur la carte Coût total Aucune donnée ASSOCIACAO DO INSTITUTO DE BIOLOGIA MOLECULAR E CELULAR Portugal Contribution de l’UE Aucune donnée Adresse Rua de Campo Alegre, 823 4150 PORTO Voir sur la carte Coût total Aucune donnée UNIVERSIDAD DE LEON Espagne Contribution de l’UE Aucune donnée Adresse AVENIDA DE LA FACULTAD 25 24071 LEON Voir sur la carte Coût total Aucune donnée University of Aberdeen Royaume-Uni Contribution de l’UE Aucune donnée Adresse 2 Tillydrone Avenue AB9 2TN Aberdeen Voir sur la carte Coût total Aucune donnée