Obiettivo Bacteria export numerous proteins across the plasma membrane and this process has been heavily exploited for protein production on an industrial scale. However, the standard 'general secretory pathway' transports proteins in an unfolded state and is inapplicable for the export of the majority of cytosolic and heterologous proteins because of their tendency to fold prematurely. Recent studies, primarily by this partnership, have revealed the operation of a novel bacterial protein export system that recognises signal pepetides bearing twin-arginine motifs (RR-SIGNAL PEPTIDES) and which has the remarkable ability to export large, fully-folded proteins. Critically, RR-signal pepetides direct the export of several heterologous proteins and this application is aimed at the exploitation of this twin-arginine translocation (Tat) system to export active, high value-added proteins from Escherichia coli and Bacillus subtilis. We propose to engineer RR-signal peptides capable of directing highly specific and efficient export in these organisms, and to produce super-exporting stains capable of exporting heterolohous proteins on a commercial scale. Campo scientifico natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-3. - Key action The "Cell factory" Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore NETHERLANDS RESEARCH SCHOOL FOR ASTRONOMY - LEGALLY REPRESENTED BY UNIVERSITY OF GRONINGEN Contributo UE Nessun dato Indirizzo 5,Broerstraat 5 9712 CP GRONINGEN Paesi Bassi Mostra sulla mappa Costo totale Nessun dato Partecipanti (7) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto ALBERT-LUDWIGS-UNIVERSITAET FREIBURG Germania Contributo UE Nessun dato Indirizzo Fahnenbergplatz 79085 FREIBURG (IN BREISGAU) Mostra sulla mappa Costo totale Nessun dato CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Francia Contributo UE Nessun dato Indirizzo CEN-Saclay 91191 GIF SUR YVETTE Mostra sulla mappa Costo totale Nessun dato GENENCOR INTERNATIONAL BV Paesi Bassi Contributo UE Nessun dato Indirizzo 139,Lange Kleiweg 139 2288 GJ RIJSWIJK ZH Mostra sulla mappa Costo totale Nessun dato JOHN INNES CENTRE Regno Unito Contributo UE Nessun dato Indirizzo Norwich Research Park, Colney NR4 7UH NORWICH Mostra sulla mappa Costo totale Nessun dato THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Regno Unito Contributo UE Nessun dato Indirizzo University Offices, Wellington Square OXFORD Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato UNIVERSITY OF WARWICK Regno Unito Contributo UE Nessun dato Indirizzo GIBBET HILL ROAD COVENTRY Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato University of East Anglia Regno Unito Contributo UE Nessun dato Indirizzo University Plain NR4 7TJ Norwich Mostra sulla mappa Costo totale Nessun dato