Objetivo We have previously successfully generated biosynthetic heparin from E. coli K5 polysaccharide using enzymatic and chemical modifications. We now want to identify new regio- specific heparan sulphate structures by screening biosynthetic ologasaccharide libraries with FGF4, FGF8 and HIV-Tat protein. Chemical and enzymatic processes will be used to generate these oligasaccharides in vitro from the K5 polysaccharide. Furthermore, both yeast and mammanlian cells, which genetically alter their biosynthetic capacity, will be used to synthesize neoGAGs. The biological efficacy of these oligosaccharides will be screened in tumour growth assays to demonstrate their antitumor activity. This can lead to new antitumor therapies. Similarly, also the HIV-Tat protein recognizing oligosaccharides will be tested for their ability to modulate the biological activity of extracellular Tat.The expert system is a windows based software which supports Experts and Non Experts during the feasibility analysis of Large Scale Solar System integration into. Default value setting is provided by databases, nomogram algorithms and calculation models. Especially Non Experts are support by the expert system through project specific default value setting. Only a view parameters are required as an input and all other parameters for system analysis are identified by the expert system. Strengths of the expert system: Wizard mode and project specific default value setting for NON-Experts Control of data input for experts through project specific default value settingOptimisation of existing systems on the basis of economic / financial criteria Technical and economic verification during data input Solar system dimensioning based on data base and nomogram algorithm Financial analysis of the entire heating system or only for the solar system User specific preparation of reports Ámbito científico natural sciencescomputer and information sciencessoftwarenatural sciencescomputer and information sciencesdatabasesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencescomputer and information sciencesartificial intelligenceexpert systemsnatural sciencesbiological sciencesbiochemistrybiomoleculescarbohydrates Programa(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Tema(s) 1.1.1.-3. - Key action The "Cell factory" Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador BIOTIE THERAPIES OY LTD Aportación de la UE Sin datos Dirección Tykistoekatu 6 20520 TURKU / ABO Finlandia Ver en el mapa Coste total Sin datos Participantes (5) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo ISTITUTO SCIENTIFICO DI CHIMICA E BIOCHIMICA "G.RONZONI" Italia Aportación de la UE Sin datos Dirección Via Giuseppe Colombo 81 20133 MILANO Ver en el mapa Coste total Sin datos RICERCHE SPERIMENTALI MONTALE SRL Italia Aportación de la UE Sin datos Dirección Via Garibaldi 33 51037 MONTALE Ver en el mapa Coste total Sin datos UNIVERSITY OF MANCHESTER Reino Unido Aportación de la UE Sin datos Dirección Oxford Road MANCHESTER Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos UNIVERSITÀ DEGLI STUDI DI BRESCIA Italia Aportación de la UE Sin datos Dirección Via Valsabbina 19 25124 Brescia Ver en el mapa Coste total Sin datos UPPSALA UNIVERSITY Suecia Aportación de la UE Sin datos Dirección Sankt Olofsgatan 10 B 75105 UPPSALA Ver en el mapa Coste total Sin datos
