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Sequencing the yeast genome - Sequence data coordination

Objectif

The objective of the informatics coordination for sequencing the yeast genome was the support of the DNA coordinators and the sequencing laboratories with a suitable, centralized informatics facility to collect, assemble, analyze and distribute the sequence of the yeast genome.
As the informatics coordinator MIPS has been responsible for the accumulation, verification, storage, and analysis of the sequence data generated by more than 50 laboratories participating in the sequencing of yeast chromosomes II, IV, VII, X, XII, XIV, XV and XVI. As of April 1996, a total of 55% of the yeast genome, equivalent to more than 6 million base pairs of non-redundant sequence were submitted to MIPS. The sequences of chromosomes II, IV, VII, X, XII, XIV, XV, and XVI have been assembled by MIPS, annotated and opened for public access. Chromosomes IV, XII, and XI have been completed in a joint effort with laboratories from Great Britain, Canada, and the United States. The final data were forwarded to the EMBL data library and the PIR-International Protein Sequence Database for public access. The European yeast sequencing effort has accomplished its mission. Earlier than expected the data have been released on April 24th, 1996. The distribution of tasks in the coordinated network of European sequencing labs between DNA coordinators, providing clones from an ordered library based on precise physical maps, the sequencing laboratories and MIPS as the informatics coordinator has been proven as reliable and efficient. The project has shown that a multi-national, decentralized approach supported by a suitable infrastructure can generate a significant impact for basic research and industrial applications. Bioinformatics plays a critical role for data assembly, verification and distribution. While increasing data throughput from 100 thousand bases in 1989 to more than 2 million per year, the procedures of data processing and annotation have been constantly improved for quality and efficiency. A frequently used service was provided to allow searches of the unpublished part of the sequence database. Preliminary confidential data were accessible for the scientific community and the members of the Yeast Industry Platform (YIP) through the submission of queries to MIPS. While coming close to the completion of the yeast genome sequence, our informatics efforts have concentrated on the comprehensive compilation of the sequence data and the associated information (annotation). The project paralleled with the advent of the World Wide Web (WWW) and its global accessibility. We have established a webserver (http://www.mips.biochem.mpg.de) that does not only allow to access and download yeast sequence data, but also provides information on functional classification, sequence homologies, structure prediction, and intragenomic gene redundancy. Information on yeast genes is updated continuously with information from the literature and related databases (e.g. SGD, Stanford; YPD, Cold Spring Harbor).

Appel à propositions

Data not available

Régime de financement

CSC - Cost-sharing contracts

Coordinateur

MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Contribution de l’UE
Aucune donnée
Adresse
Am Klopferspitz 18 A
82152 MARTINSRIED
Allemagne

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Coût total
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