Objetivo The goal of this project is to develop novel expression systems and mutant transgenic organisms, in order to gain insights into the role of the 5q31 region, revealing the functions of genes involved in haematopoiesis and the leukaemia processes. To this end, we will model aspects of the pre-leukemic 5q- deletion-mutation syndrome in the mouse, by using a series of homologous recombination and novel expression systems strategies. More specifically, based on a previously successful technological approach, we plan to 1) delete in the mouse the synthetic candidate 'critical region' between the GM-CSF /IL3 and TCF1 genes, included in our constructed human YAC contig. Our approach will utilize the Cre-IoxP recombination system combined with conditional targeting, to induce the deletion of the 5q31 region in the adult mouse myeloid cells only (like in humans), by linking the Cre recombines gene, under the myeloid and adult-specific promoter of the myeloperoxidase gene. Thus, the generated animal models containing precise large chromosomal deletions, occurring as acquired somatic mutations, will accurately mimic the human pre-leukaemia syndrome 2) Systematically analyse the resulting phenotypes of the mice by differential display technology approaches, to reveal the deregulated pathways and gene networks occurring in these models Ámbito científico natural sciencesbiological sciencesgeneticsmutationmedical and health sciencesclinical medicineoncologyleukemia Programa(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Tema(s) 1.1.1.-8. - Research into genomes and diseases of genetic origin Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador FOUNDATION OF RESEARCH AND TECHNOLOGY - HELLAS Aportación de la UE Sin datos Dirección Vassilika Vouton 71110 IRAKLION Grecia Ver en el mapa Coste total Sin datos Participantes (4) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo HUMBOLDT-UNIVERSITAET BERLIN Alemania Aportación de la UE Sin datos Dirección 20-21,Schumanstrasse 20-21 10117 BERLIN Ver en el mapa Coste total Sin datos INSTITUTE OF CANCER RESEARCH : ROYAL CANCER HOSPITAL Reino Unido Aportación de la UE Sin datos Dirección Old Brompton Road 123 SW7 3RP LONDON Ver en el mapa Coste total Sin datos KLINIKUM DER FRIEDRICH-SCHILLER-UNIVERSITAET JENA Alemania Aportación de la UE Sin datos Dirección Bachstrasse 18 07740 JENA Ver en el mapa Coste total Sin datos THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Reino Unido Aportación de la UE Sin datos Dirección Roosevelt Drive OX3 7BN OXFORD/HEADINGTON Ver en el mapa Coste total Sin datos
