Skip to main content
European Commission logo print header

Cloning of zebrafish craniofacial mutations. Their function in morphogenesis

Objetivo

We propose a detailed analysis of two zebrafish mutants with craniofacial and heart defects. Our goal is to isolate and characterize the mutated genes that are crucial for vertebrate development. The immediate goal for these two years is to map and clone the mutations and perform an extensive analysis of mutant phenotypes using in situ hybridizations, histological analysis and transplantation experiments. We will attempt to positionally clone the mutated genes using modern molecular genetic tools and taking advantage of the powerful techniques of zebrafish genetics. If novel genes are thus identified, their mammalian homologues will then be sought by in silico screening of EST databases. Molecular characterization of the genes will be completed and we will micro-inject DNA to mutant embryos to rescue the phenotype. We hope that this work will ultimately lead to a better understanding of the molecular and cellular basis of congenital human diseases of craniofacial structures and valve malformations.

Convocatoria de propuestas

Data not available

Coordinador

Forschungzentrum für Umwelt und Gesundheit GmbH
Aportación de la UE
Sin datos
Dirección
Ingolstädter Landstrasse 1
85764 Neuherberg
Alemania

Ver en el mapa

Enlaces
Coste total
Sin datos