Dna recognition and photophysical properties of groove-located dinuclear transition metal complexes

Ziel

Understanding, on a molecular level, how genetic information is expressed and how to stimulate or prevent this process is a key step towards the development of new chemotherapeutic strategies. In this framework, dimine transition metal complexes present particularly well-suited photophysical properties to develop new DNA photoprobes. Dinuclear iron (II) and ruthenium (II) complexes have been recently shown to interact very strongly within the DNA grooves and are probably the most promising candidates for such applications. The aim of the project is to deepen the knowledge about the photophysical properties and the interaction with DNA of this class of compounds. More precisely, sequence-selective recognition will be explored through fictionalization of their ligands. Two complementary research approaches will be developed: 1. The in-depth study (including both thermodynamic and kinetic aspects) of the effects of fictionalization of these dinuclear complexes on their interaction with DNA, especially concerning sequence discrimination. 2. The detailed study of the photophysical properties of these fictionalized compounds (including energy and electron transfer between the metal centres) in solution and in the presence of DNA. In both cases, the combination of different powerful spectroscopic methods including linear dichroism and luminescence measurements will be used. Quantum mechanical calculations are also planned to support experimental results interpretation.

Wissenschaftliches Gebiet

Programm/Programme

FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Thema/Themen

Data not available

Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

RGI - Research grants (individual fellowships)

Koordinator

CHALMERS UNIVERSITY OF TECHNOLOGY

EU-Beitrag

Keine Daten

Adresse

3,Kemivagen 10
412 96 GOETEBORG
Schweden

Gesamtkosten