Objective The present project is focused on the evaluation of the adhesive surface properties under conditions of contact with the bio-medium and biocompatibility of nano-layered diamond-like carbon (DLC), carbon-containing (CC) films and multi-component nano-structured thin films deposited by PVD and CVD for various biomedical applications. The DLC and CC films will be formed by the direct ion-plasma flows of hydrocarbon vapors and by plasma chemical deposition. Nano-structured thin films based on the system Ti-X-Y (X=Al,Si,V,Zr,Nb, and Ca; Y=C,O, and N) will be deposited using unbalanced magnetron sputtering (UBMS) and ionised PVD (I-PVD) of composite targets. The work will encompass(i) the development and fabrication of new composite targets by self-propagating high-temperature synthesis (SHS);(ii) the subsequent deposition of composite thin films from these target using UBMS (PVD) and I-PVD;(iii) analysing the ionised fraction of metal atoms for different PVD parameters and modelling of the ionisation mechanisms for different elements;(iv) the microstructural evolution of the thin films (using TEM, HRTEM, SFM, SEM, SIMS, AES and XPS) as a function of ionised fraction of metal and non-metal atoms and various PVD and CVD processing parameters, and;(v) the evaluation of the properties (adhesion, microhardness, roughness, density, toughness, elasticity, wear- and corrosion resistance, surface charge) of the thin films. The shape/morphology, modes of locomotion and cytoskeletal organizations of the two main types of tissue cells, fibroblasts and epitheliocytes, cultivated on various nanostructured substrata will be analysed.The biocompatibility of the films will be evaluated by both in vitro and in vivo experiments. In vitro studies will involve the investigation of the adhesion of fibroblasts or epithelial cells to the films, morphometric analysis of the cells cultivated on the films, and immunofluorescence microscopic investigations of actin cytoskeleton in cells on the surface of tested films. In vivo studies will be fulfilled by subcutaneous implantation of plates coated with the tested films to mice in order to reveal the possible inflammatory reaction in the animals. The development of cell cultures for different nano-crystalline distributions will be studied and the relationship between nano-structured properties and biocompatibility will be established. The antibacterial activity and chemical inertness of the films in various biomedia will be achieved.The proposed research project is interdisciplinary and the research program will be conducted jointly between six research teams: Joint Research Centre, Institute for Health and Consumer Protection (CO), University of Paris, Orsay (CR1), Centre Hospitalier Universitaire in Nice (CR2), Moscow Steel and Alloys Institute (CR3), Moscow State Aviation Technological University by K.E. Tsiolkovsky (CR4) and Cancer Research Centre of the Russian Academy of Medical Science (CR5). Each of these institutions provides a complementary range of expertise and research facilities that includes material scientists (CO, CR3), plasma processing and deposition experts (CO, CR1, CR4) as well as biologists (CR2, CR5). Research funds are being requested from the INTAS program to support the research that will be performed by CR3, CR4 and CR5 personal in Moscow (80%), while the research that will be performed at CO, CR1 and CR2 will be largely funded through industrial support and national research programs. Some 20% of INTAS funds will be used to supplement the travel expenses needed to facilitate the travel of the NATO team researchers to Moscow, labour costs for coordination and as a lump sum for management. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Topic(s) 1B - Condensed Matter, Optics and Plasma Physics NANO - NANO : Nano-scale Materials and Structures Call for proposal Data not available Funding Scheme Data not available Coordinator Joint Research Center of European Commission EU contribution No data Address Via E. Fermi 1 21020 Ispra (Va) Italy See on map Total cost No data Participants (5) Sort alphabetically Sort by EU Contribution Expand all Collapse all Moscow State Aviation Technological University by K.E. Tsiolkovsky Russia EU contribution No data Address 27 Petrovka Street 103767 Moscow See on map Total cost No data Russian Academy of Medical Sciences Russia EU contribution No data Address Kashirscoye Shosse 24 115487 Moscow See on map Total cost No data Technological University Russia EU contribution No data Address Leninsky pr. 4 119991 Moscow See on map Total cost No data Universite de Paris France EU contribution No data Address Batiment 210 91405 Paris See on map Total cost No data University of Nice Sophia Antipolis France EU contribution No data Address 30 Av. de la Voie Romaine 06025 Nice See on map Total cost No data
