Objectif To minimize the risk of nosocomial respiratory infection caused by P. aeruginosa it is planned:-to screen existing P. aeruginosa phage collection of the IBMV(GE) comprised of 30 clones against about 200 strains obtained from the IECS (GE) clinic;-to isolate up to 20 new phage clones from environment;-to select up to 10 candidate phases on the base of the their biological features and genetic character of the host-phage interaction;-to investigate gene structure of the best 5 candidate phages with the purpose to avoid possible spread of imipenem drug-resistance;-to perform clinical experiments comprising phage treatment of hospital environment of the Department of Lung Surgery in the IECS (GE);-to elaborate a new inexpensive and efficient strategy based on application of specific bacteriophage(s) for prevention of P. aeruginosa nosocomial infection, which will result in a reduction in the cost of prophylaxis.Research activities:For realization of the goals of the project the following research activities shall be accomplished:1) Microbiology studies of the clinical environment and patients;2) Construction of the strain collection by using standard (host) strains from the ATCC and other collections and newly isolated and studied of nosocomial Pseudomonas strains;3) Screening the strains for phage-sensitivity against the IBMV phage collection and phage clones isolated from environment during the present study;4) Selection of candidate phage(s) and appropriate host(s);5) Characterization of the candidate phages which involves the following studies:a)Morphological and biological studies;b)Study of the efficiency of plating (EOP);c)Resistance of phages towards physical and chemical agents;d)Serological relatedness of the phages;e)Intracellular phage growth cycle (IPGC);f)Ability of phages to transduce drug-resistance;g)Characterization of the phage DNA and determination of thegenetic relatedness of phages;6) Genetic studies of the host-strains which involves the following stages:a) Detection of plasmids;b) Detection of temperate phages;c) Study of the frequency of occurrence of mutations to phage-resistance (PRM);7.Extension of the host-range of the selected phages and creation of the complex phage preparations with broad spectrum of activity;8. Experiments for assessment the prophylactic efficiency of Pseudomonas phage;9. Analysis of obtained results and their statistical assessment. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) 4 - Life Sciences GEORGIA - GEORGIA Appel à propositions Data not available Régime de financement Data not available Coordinateur Institute for Animal Health Contribution de l’UE Aucune donnée Adresse Compton RG20 7NN Newbury Royaume-Uni Voir sur la carte Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Institute of Bacteriophage, Microbiology and Virology Géorgie Contribution de l’UE Aucune donnée Adresse Gotua Street 3 380060 Tbilisi Voir sur la carte Coût total Aucune donnée Institute of Experimental and Clinical Surgery (IECS) Géorgie Contribution de l’UE Aucune donnée Adresse Chachava Street 4 380059 Tbilisi Voir sur la carte Coût total Aucune donnée Laboratory of Microbial Molecular Genetics France Contribution de l’UE Aucune donnée Adresse Route de Narbonne 118 31062 Toulouse Voir sur la carte Coût total Aucune donnée