Cel Genetic function and molecular organization of heterochromatin still remains largely elusive. In order to delucidate the genetic and molecular nature of this peculiar genomic component new approaches and tight cooperation of specialists in different fields are required.The discovery of a new gene, named Su(UR)ES, controlling the degree of underreplication of pericentric heterochromatin provides a good opportunity to study the gene involved in the process of incomplete polytenization and to characterize the newly polytenized portions of heterochromatin. Cloning the gene, sequencing, and expression analysis will be performed in order to understand the genetic mechanisms underlying polytenization (underreplication) of both pericentric and intercalary heterochromatin.Microdissection and cloning of portions of pericentric heterochomatin that polytenize in Su(UR)ES mutants will provide a new approach to the molecular analysis of heterochromatic regions which are usually cytologically "invisible" in all Drosophila stocks. Moreover, the newly isolated heterochromatic portions will be used as additional markers of pericentric heterochromatin. This will permit to construct new, highly resolutive map of pericentric heterochromatin. For these purposes a set of breakpoints of chromosomal rearrangements will be simultaneously mapped on mitotic and polytene heterochromatin maps.Preliminary data from microclone analysis show that newly polytenized heterochromatic regions are enriched in I elements and also contain about 20 unique sequences. Analysis of I-element distribution in the Su(UR)ES as well as several strains from the wild will be performed.Finally, the use of the unique sequences as probes will allow the recovery of long stretches of heterochromatic DNA from BAC, P1 cosmids, and L phage libraries, and to study the molecular organization of these DNA sequences in known regions of heterochromatin. Program(-y) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Temat(-y) 4 - Life Sciences OPEN - OPEN Call Zaproszenie do składania wniosków Data not available System finansowania Data not available Koordynator University of Rome, " La Sapienza" Wkład UE Brak danych Adres 24 Piazzale A. Moro 5, 00185 Rome Włochy Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (4) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Russian Academy of Sciences Rosja Wkład UE Brak danych Adres Vavilov street 34/5 117334 Moscow Zobacz na mapie Koszt całkowity Brak danych Siberian branch of Russian Academy of Sciences Rosja Wkład UE Brak danych Adres Lavrentiev prospect, 10 630900 Novosibirsk Zobacz na mapie Koszt całkowity Brak danych State University of Novosibirsk Rosja Wkład UE Brak danych Adres Pirogov Street, 2 630090 Novosibirsk Zobacz na mapie Koszt całkowity Brak danych Université de Genève Szwajcaria Wkład UE Brak danych Adres 30 quai Ernest Ansermet 1211 Geneva Zobacz na mapie Koszt całkowity Brak danych
