Objetivo The project aims at identifying the molecular components of the genetic networks controlling the architecture, growth and shape of leaves by using Arabidopsis as a model for simple leaf formation and its close relative Cardamine as a model for compound leaf formation. In Arabidospsis a set of 94-leaf mutant will form the core for high throughput positional cloning of growth and regulatory genes by using AFLP, INDEL and SNP molecular marker technology. Differential cDNA display on the leaf -inducible pin 1-1 Arabidopsis shoot apex will identify leaf architecture genes. A set of knockout and activation tagged Arabidopsis lines is available and will be screened for additional leaf phenotypes, some of the sequences of the insertion sites will be used for reversed genetics. The regulatory pathway controlled by the myb transcription factor, mybF23N20, with specific expression in the leaves will be analyzed by using functional genomics tools. Genes that control compound leaf formation will be identified either in mutant screens or from the analysis of the KNOX pathway in Cardamine. The genetic networks themselves will be unraveled by investigation of the gene interactions at the genetic level. A model for growth in leaves using several parameters will be determined for simple and compound leaf wild types, which will enable to classify the mutants according to their growth defects. Genome-wide gene expression analyses will be performed on wild type and mutants at different growth stages by micro-array analysis in order to describe the molecular pathways that are acting during a specific growth stage or that are induced or inactivated upon mutation. A knowledge database will be generated for the in depth study of genotype-phenotype relationships and will enable to build genetic models on leaf architecture, leaf growth and leaf shape. Genes will be selected from these studies to test their utility to improve leaf growth in the distantly related crop rice a Ámbito científico natural sciencescomputer and information sciencesdatabasesnatural sciencesbiological sciencesgeneticsmutationagricultural sciencesagriculture, forestry, and fisheriesagriculture Programa(s) FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002) Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación NET - Research network contracts Coordinador FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW Aportación de la UE Sin datos Dirección Ledeganckstraat 35 9000 GENT Bélgica Ver en el mapa Coste total Sin datos Participantes (5) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo CROPDESIGN N.V. Bélgica Aportación de la UE Sin datos Dirección Technologiepark 3 9052 ZWIJNAARDE Ver en el mapa Coste total Sin datos INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE Francia Aportación de la UE Sin datos Dirección Place Pierre Viala 2 34060 MONTPELLIER Ver en el mapa Coste total Sin datos JOHN INNES CENTRE Reino Unido Aportación de la UE Sin datos Dirección Colney Lane - Norwich Research Park NR4 7UH NORWICH Ver en el mapa Coste total Sin datos THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Reino Unido Aportación de la UE Sin datos Dirección SOUTH PARKS ROAD OX1 3RB OXFORD Ver en el mapa Coste total Sin datos UNIVERSITY OF BERNE Suiza Aportación de la UE Sin datos Dirección Altenbergrain, 21 3012 BERN Ver en el mapa Coste total Sin datos
