Obiettivo This project aims to develop a protocol for effective immunotherapy of breast and ovarian cancer based on a prime boost strategy and the MUC1 antigen which is over-expressed and aberrantly glycosylated in 90% of these malignancies. The strategy will combine cDNA priming with a glycoprotein boost. Different cancer-associated glycoforms of MUC1 will be produced in CHO cells, wild type, and modified by transfection of specific glycosyl transferases. The glycoproteins will be purified, characterised and comparatively evaluated with MUC1 cDNA for efficacy in tumour rejection in mouse models. Chemo-enzymically synthesised MUC1 glycopeptides will be used to evaluate immune responses. Conditions for production to GMP standard of MUC1 cDNA and the selected glycoprotein will be established to prepare for a clinical study. The pattern of glycosylation in cancer cells will be defined with microarray technology and MUC1 cDNA modified. Campo scientifico medical and health sciencesclinical medicineoncologymedical and health sciencesbasic medicineimmunologyimmunotherapynatural sciencesmathematicspure mathematicsarithmeticsprime numbers Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-3. - Key action The "Cell factory" Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore CANCER RESEARCH UK Contributo UE Nessun dato Indirizzo Lincoln's Inn Field 61 WC2A 3PX LONDON Regno Unito Mostra sulla mappa Costo totale Nessun dato Partecipanti (7) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CANCER RESEARCH TECHNOLOGY LIMITED Regno Unito Contributo UE Nessun dato Indirizzo Dominion House, St-Bartholomew's Hospital, 59 Bart EC1A 7BE LONDON Mostra sulla mappa Costo totale Nessun dato FORSCHUNGSZENTRUM JUELICH GMBH Germania Contributo UE Nessun dato Indirizzo 52425 JUELICH Mostra sulla mappa Costo totale Nessun dato GOETEBORG UNIVERSITY Svezia Contributo UE Nessun dato Indirizzo 9,Medicinaregatan 9 405 30 GOETEBORG Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF COPENHAGEN Danimarca Contributo UE Nessun dato Indirizzo 20,Noerre Allee 20 2200 KOEPENHAGEN Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF ROME "LA SAPIENZA" Italia Contributo UE Nessun dato Indirizzo Viale Regina Elena 324 00161 ROMA Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF UMEAA Svezia Contributo UE Nessun dato Indirizzo 90187,Universitetsomradet, Naturvetarhuset 901 57 UMEAA Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF ZURICH Svizzera Contributo UE Nessun dato Indirizzo 30,Gloriastrasse 30 8028 ZURICH Mostra sulla mappa Costo totale Nessun dato