Objectif Understanding how the regulation Beta1 integrin is achieved is a very active research field. This control likely involves a direct binding of a cytoplasmic protein with the integrin. I have shown using in vitro and cell culture approach that ICAP-1 (Integrin Cytoplasmic domain Associated Protein) negatively regulates Beta1 integrin function. With my proposal I will use knockout technology to unravel ICAP-1 function during development. To achieve this goal I have joined an outstanding laboratory in this field. I will generate two different mouse strains. The first one will be a complete knockout of the gene, while the second will be an exon specific deletion within the ICAP-1 gene. The deieted exon encodes the integrin binding site, and these mice carrying such a mutation will be useful to ask whether ICAP-1 interaction is essential in vivo. Both targeting vectors have been already electroporated into ES cells. I will start the phenotype analysis of these mice next year. This will be done using cell biology, biochemistry, histology and immunochemistry. Altogether these different approaches will give a deep insight into ICAP-1 function and will provide a better understanding of the regulation of Beta1 integrin functions. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencescell biologynatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological scienceshistology Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Thème(s) 1.1.1.-8. - Research into genomes and diseases of genetic origin Appel à propositions Data not available Régime de financement RGI - Research grants (individual fellowships) Coordinateur LUND UNIVERSITY Contribution de l’UE Aucune donnée Adresse Universitetssjukhuset 221 85 LUND Suède Voir sur la carte Coût total Aucune donnée
