Objetivo We propose to analyse the synaptic integration in the mistral cell dendrites during synaptic activation by electrophysiological recordings and simultaneous calcium imaging at the single cell level. The long-term goal is to combine cellular and network experiments in the analysis of the mistral cell dendrite function during network activity, using the turtle olfactory bulb at physiological temperature. The analysis will be facilitated by the ability to record from the distal dendrites electrophysiological events, which may occur distally, while only small responses are observed in recordings from the cell body. Recordings from the dendrites will be combined with stimulation of the olfactory nerve (ON). This allows a precise analysis of the ON EPSP, including the response as function of presynaptic firing frequency, and gives estimation of the number of presynaptic axons needed to evoke local vs. somatic spikes. ON stimulation or mistral cell dendrite spiking is followed by local inhibitory synaptic activity in the tuft. Minimal, focal ON activation and periglomerular stimulation will evoke Pips in the mistral cells, which can be compared with the Pips due to dendro-dendritic feedback following postsynaptic spiking in the mistral cell recorded from. After the electrophysiological experiments, the distribution of the dendrite calcium concentration during ON activation will be analysed by simultaneous whole cell recording and calcium imaging. The mistral cell de Ámbito científico natural sciencesbiological sciencesneurobiologynatural scienceschemical sciencesinorganic chemistryalkaline earth metals Programa(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Tema(s) 1.1.1.-9. - Neurosciences Convocatoria de propuestas Data not available Régimen de financiación RGI - Research grants (individual fellowships) Coordinador UNIVERSITY OF COPENHAGEN Aportación de la UE Sin datos Dirección Blegdamsvej 3 2200 KOEPENHAGEN Dinamarca Ver en el mapa Coste total Sin datos
