Objective Salmonella in broiler poultry remains the major source of infection for man and is the least tractable because of the young age at which broilers are slaughtered and their immunological immaturity. In previous project (FAIR 98-4006) we have found that intestinal colonisation of newly hatched chicks with live, attenuated Salmonella vaccine strains results in the development of a rapid and profound resistance to intestinal infection (from a specific microbiological exclusion mechanism) and also to tissue invasion (from an induction of heterophil infiltration into the intestine). We will exploit these phenomena by screening strains of Salmonella stereotypes Typhimurium, Entertains and Hagar for these phenotypes, introducing attenuating mutations and assessing their protective effect in broilers under experimental and simulated field conditions. The degree of attenuation induced and the host cell responses to the vaccines will also be studied. In addition, we will explore the potential of new biotechnological approaches for the elimination of pathogenic bacteria (wild-type and vaccine strains) from the chicken intestine bya) identifying colonisation genes in Salmonella and mutating them,b) exploring the potential of introducing inducible bacterial suicide genes into Salmonellac) studying hostdefensin production in the intestine and its induction by vaccinesd) dietary manipulation to modulate expression of invasion genes. Microarray technology will be used to identify colonisation andheterophil-induction genes and examine host responses. We will also exploit our expertise in phage technology to explore the use of bacteriophages to control intestinal infection and reduce carcass contamination by Salmonella and use these in association with vaccine strains totes a combined approach to pathogen elimination. Fields of science natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesmicrobiologyvirologymedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccinesnatural sciencesbiological sciencesgeneticsmutationmedical and health sciencesclinical medicinegastroenterology Keywords Chicken Food Food-safety Immunity Salmonella safety Programme(s) FP6-FOOD - Food Quality and Safety: Thematic priority 5 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) FOOD-2002-T3 - Total food chain: Pathogen free production systems Call for proposal FP6-2002-FOOD-1 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator INSTITUTE FOR ANIMAL HEALTH EU contribution No data Address - COMPTON United Kingdom See on map Total cost No data Participants (12) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITEIT GENT Belgium EU contribution No data Address Sint Pietersnieuwstraat 25 GENT See on map Total cost No data FRIEDRICH-LÖFFLER-INSTITUT, FEDERAL RESEARCH CENTER FOR VIRUS DISEASES OF ANIMALS Germany EU contribution No data Address Boddenblick 5 A GREIFSWALD See on map Total cost No data VETERINARY RESEARCH INSTITUTE Czechia EU contribution No data Address Hudcova 70 BRNO See on map Total cost No data VETERINARY MEDICAL RESEARCH INSTITUE - HUNGARIAN ACADEMY OF SCIENCES Hungary EU contribution No data Address Hungaria krt. 21 BUDAPEST See on map Total cost No data LOHMANN ANIMAL HEALTH GMBH & CO. KG Germany EU contribution No data Address Heinz-Lohmann-Strasse 4 CUXHAVEN See on map Total cost No data INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE France EU contribution No data Address Rue de l'Universite 147 PARIS See on map Total cost No data ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V. Netherlands EU contribution No data Address Edelhertweg 15 LELYSTAD See on map Total cost No data UNIVERSITY OF BRISTOL United Kingdom EU contribution No data Address Senate House, Tyndall Avenue BRISTOL See on map Total cost No data EBI B.V. Netherlands EU contribution No data Address De Savornin Lohmanlaan 224 DEN HAAG See on map Total cost No data THE UNIVERSITY OF NOTTINGHAM United Kingdom EU contribution No data Address University Park NOTTINGHAM See on map Total cost No data STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK Netherlands EU contribution No data Address Costerweg 50 9101 WAGENINGEN See on map Total cost No data EBI FOOD SAFETY BV Netherlands EU contribution No data Address Nieuwe Kanal 7P WAGENINGEN See on map Total cost No data
