Objectif NEONUCLEI will develop transcription-competent synthetic analogues of cell nuclei. These particles, termed neonuclei, will be obtained through self-assembly/organisation in mixtures of DNA, macromolecules (or nanoparticles), and lipids. The composition o f the neonuclei will be chosen to produce particles with internal nano-architectures capable of sustaining gene transcription upon the addition of transcription factors. These design of the static architectures of the neonuclei and their dynamic propert ies will be guided by observations on real nuclei. The DNA of the neonuclei will contain a gene cluster (or tandem repeats of the same gene). The genes will be separated by sequences designed to induce DNA compaction in response to specific chemical or p hysical stimuli. This will be exploited to establish non-biological control over the transcription of parts, or all, of the DNA. These control sequences offer the opportunity for multiple transcription control strategies and provide the capability of im plementing temporally co-ordinated synthesis of multiple gene products. Neonuclei represent a key enabling step in the realisation of semi-biotic systems; these are systems and devices that combine synthetic non-natural functional systems with systems o f biological origin. The neonuclei will be integrated with biological systems, or with isolated components, to produce novel semi-biotic devices capable of the controlled in situ synthesis of complex bio-molecules on demand. Champ scientifique natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsengineering and technologynanotechnologynano-materials Mots‑clés Nanotechnology Risk Assessment Programme(s) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) NEST-2003-1 - Adventure activities Appel à propositions FP6-2003-NEST-PATH Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur UNIVERSITY OF SOUTHAMPTON Contribution de l’UE Aucune donnée Adresse University Road, Highfield SOUTHAMPTON Royaume-Uni Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire LUNDS UNIVERSITET Suède Contribution de l’UE Aucune donnée Adresse Paradisgatan 5c LUND Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée CHALMERS TEKNISKA HOGSKOLA AB Suède Contribution de l’UE Aucune donnée Adresse Chalmersplatsen 4 GOTEBORG Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSIDADE DE COIMBRA Portugal Contribution de l’UE Aucune donnée Adresse HospitaIS da Universidade de Coimbra - Praceta Mota Pinto COIMBRA Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN Allemagne Contribution de l’UE Aucune donnée Adresse Geschwister-Scholl-Platz 1 MUENCHEN Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée
