Objectif Implementing highly parallel cell based assays able to replace animal testing is crucial for a large variety of industries. Some cell-based assays have already been achieved using multi-wells plastic plates where individual cell cultures and their stimulations are realised in wells. Unfortunately these novel technologies did not allow to get the level of discoveries that was expected when phenotyping cells for drug discovery and toxicology. DNA chips experiments have already permit massive analyses of animal and human genomes particularly used for in vitro toxicological studies. More information could be obtained replacing the DNA spots by drops of cell culture allowing to establish the relationship between cell phenotypes and chemical toxicity. The aim of this two year ToxDrop project is to develop a Cell on Chip technology where various phenotypes illustrating cytotoxicity can be characterised through the phenotyping of cells in highly parallel cell based reactions on a single chip. The substrate of the biochip will allow to achieve the necessary precision in the formation of the cellular nanodrops and the high content analyses of cell cultures based on fluorescent, high contrast optical based detection as well as mass spectrometry phenotypic signatures. The project will help three European SMEs to get access to the pioneer technology of the Cell on Chip which could be used in particular for cell based in vitro toxicological studies by the industry. The ToxDrop validation will be planned through discussions with ECVAM and realised through tests in cellular nanodrops by academic laboratories specialised in pharmacological and chemical toxicological studies. In order to reproduce on the Cell on Chip several assays previously tested in animals, toxicity will be carried out in nanodrops containing several types of cells issued from various organs of several species. In particular some problems never solved without the use of animal will be tackled... Champ scientifique medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverynatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsmicroscopynatural scienceschemical sciencesanalytical chemistrymass spectrometrymedical and health sciencesbasic medicinetoxicology Mots‑clés Cell On chip Nanotechnology cell based phenotyping cytotoxicity gene transfection microarray non-animal testing siRNA Programme(s) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) POLICIES-2.2 - Public health issues, including epidemiology contributing to disease prevention and responses to emerging rare and communicable diseases, allergies, procedures for secure blood and organ donations, non-animal test methods Appel à propositions FP6-2003-SSP-3 Voir d’autres projets de cet appel Régime de financement STIP - Specific Targeted Innovation Project Coordinateur COMMISSARIAT À L'ENERGIE ATOMIQUE Contribution de l’UE Aucune donnée Adresse 17 rue des martyrs cedex9 GRENOBLE France Voir sur la carte Coût total Aucune donnée Participants (7) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE France Contribution de l’UE Aucune donnée Adresse 101 rue de Tolbiac PARIS Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITÉ CLAUDE BERNARD LYON 1 France Contribution de l’UE Aucune donnée Adresse 43 Boulevard du 11 novembre 1918 VILLEURBANNE Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée ECOLE POLYTECHNIQUE FÉDÉRALE DE LAUSANNE Suisse Contribution de l’UE Aucune donnée Adresse EPFL LAUSANNE Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée IMSTAR France Contribution de l’UE Aucune donnée Adresse RUE NOTRE DAME DES CHAMPS, 60 PARIS Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée TASCON GESELLSCHAFT FÜR OBERFLÄCHEN- UND MATERIALCHARAKTERISIERUNG MBH Allemagne Contribution de l’UE Aucune donnée Adresse Mendelstrasse 11 MÜNSTER Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée VITAMIB SARL France Contribution de l’UE Aucune donnée Adresse 29 BOULEVARD DES ALPES MEYLAN CEDEX Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée JOINT RESEARCH CENTRE (JRC) Belgique Contribution de l’UE Aucune donnée Adresse RUE DE LA LOI 200 BRUSSELS Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée