Obiettivo Molecular diagnostics of microbial pathogens is an integral part of modern medicine. The growing need for direct genotyping and/or the screening of the transcriptome calls for the development of alternative technologies. The STREP consortium plans to devel op a cost-effective platform for the identification bacterial species based on the SLIC-Nanobiosystem. Using tmRNA transcripts of the bacterial ssrA gene, we will be able to detect, quantify and identify bacterial species in a single homogenous assay forma t. The SLIC-Nanobiosystem consists of a self-assembled lipid bilayer membrane that integrates a synthetic ligand-gated ion channel (SLIC). The SLIC comprises a capture molecule that can specifically bind a given analyte, a process that is monitored via ele ctrical impedance spectroscopy. With this system the effect from even a few channels can be resolved thus providing an ultra-sensitive, highly stable and versatile biosensor platform. We intend to employ transcripts (tmRNA) of the ssrA gene in order to ide ntify bacterial species present in clinical samples. These transcripts occur in high abundance and contain a core sequence that is species specific, a feature which will be exploited to identify infectious disease pathogens. Identification of the different bacterial tmRNA transcripts will be accomplished by displaying a library of nucleic acid capture probes on the SLIC. This will enable species identification and discrimination between one or more species present in the sample if mixed species infection is present. Since the detection equipment will be based on electronics, the realization of miniaturized/compact and cost-effective instruments will be possible. Our approach will lay the foundation for a new generation of multiparametric molecular testing sy stems, that will open novel opportunities within the area of point-of-care applications in the clinical diagnostics market. Campo scientifico medical and health sciencesbasic medicinepharmacology and pharmacydrug discoveryengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsnatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsRNA Parole chiave Bionanotechnology Biosensors Molecular diagnostics Nanotechnology Programma(i) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) LSH-2003-1.2.5-4 - New bioassays and biosensors using post-genomic approaches for detection of harmful microbes Invito a presentare proposte FP6-2003-LIFESCIHEALTH-I Vedi altri progetti per questo bando Meccanismo di finanziamento STIP - Specific Targeted Innovation Project Coordinatore AYANDA BIOSYSTEMS Contributo UE Nessun dato Indirizzo PSE Parc Scientifique EPFL LAUSANNE Svizzera Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (5) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE Svizzera Contributo UE Nessun dato Indirizzo EPFL LAUSANNE Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato NATIONAL UNIVERSITY OF IRELAND, GALWAY Irlanda Contributo UE Nessun dato Indirizzo University Road GALWAY Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato ESTONIAN BIOCENTRE Estonia Contributo UE Nessun dato Indirizzo Riia, 23b TARTU Mostra sulla mappa Costo totale Nessun dato EURELATIONS AG Svizzera Contributo UE Nessun dato Indirizzo Technoparkstr. 1 ZURICH Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato ALBERT LUDWIGS UNVERSITÄT FREIBURG Germania Contributo UE Nessun dato Indirizzo George Koehler 103 FREIBURG Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato
