Objective Chirality is a key factor in the efficacy of many drugs and the production of single enantiomers of chiral intermediates has therefore become increasingly important. Biocatalysis offers high enantioselectivity and regioselectivity in chiral synthesis through enzyme-catalyzed reactions and thus has an important advantage over chemical synthesis. Molecular genomic data is an unprecedented resource of enzymes for biocatalysis, but rational and effective methodologies must be established to realize the full potential of these resources. This project will focus on the discovery of novel enzymes, from both public and proprietary eubacterial genomes, in particular novel alcohol dehydrogenases, cytochrome P450 monooxygenases and amino acid modifying enzymes for use in established and innovative processes for chiral synthesis.The DATAGENOM project extends from genome analysis, through cloning, expression, enzyme production, screening and protein engineering, to the enzymatic production of chiral biomolecules. The design of the project takes advantage of broad funnel-approach starting with innovative data-mining and processing of a large number of genes to ensure high flow- through in the process and rational selection of best enzyme candidates. The specific combination of expertise and design of the research project is aimed at high success-rate for the development of successful biocatalysts. Emphasis will be put on effective bioinformatics analysis to minimize the requirement for the more laborious "wet chemistry" analysis as well as development of optimised vector-host systems for efficient gene expression and enzyme production. Rational protein engineering or directed molecular evolution will be employed in order to obtain more robust variants, new substrate preferences or enhanced enantiomeric selectivity. Fields of science natural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencescatalysisbiocatalysisnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Keywords Genomes chiral biomolecules enzymes metagenome Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2002-1.2.5-4 - Exploiting post genomics to produce optically active therapeutic biomolecules by biocatalysis Call for proposal FP6-2002-LIFESCIHEALTH See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator PROKARIA EHF EU contribution No data Address Gylfaflot 5 REYKJAVIK Iceland See on map Total cost No data Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all INGENZA LTD. United Kingdom EU contribution No data Address Caslte street, 39 EDINBURGH See on map Total cost No data LUNDS UNIVERSITET Sweden EU contribution No data Address Paradisgatan 5c LUND See on map Total cost No data UNIVERSITAET STUTTGART Germany EU contribution No data Address Keplerstrasse 7 STUTTGART See on map Total cost No data UNIVERSIDAD DE OVIEDO Spain EU contribution No data Address Plaza de Riego no 4 OVIEDO See on map Total cost No data DEGUSSA AG Germany EU contribution No data Address Benningsenplatz 1 HANAU-WOLFGANG See on map Total cost No data BIOINFOBANK INSTITUTE Poland EU contribution No data Address Limanowskiego 24/a POZNAN See on map Links Website Opens in new window Total cost No data JFC - JUELICH FINE CHEMICALS GMBH Germany EU contribution No data Address Rudolf-Schulten-Strasse 5 JUELICH See on map Total cost No data CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL Germany EU contribution No data Address OLSHAUSENSTRASSE 40 KIEL See on map Total cost No data