Objectif The nematode Caenorhabditis elegans is a widely appreciated, powerful platform in which to study important biological mechanisms related to human health. More than 65% of human disease genes have homologues in the C. elegans genome, and essential aspects of mammalian cell biology, neurobiology and development are faithfully recapitulated in this organism. We propose to develop cutting-edge tools and resources that will facilitate modeling of human pathologies in C. elegans, and advance our understanding of animal development and physiology. Specifically, we will optimize and automate existing transposon-mediated mutagenesis methodologies based on the Mos1 transposable element, in addition to developing alternatives using the Minos transposon system. We will exploit these tools to generate and evaluate a collection of transposon-tagged mutants, aiming to cover the complete genome of approximately 20,000 genes. Further, we will establish the infrastructure necessary for managing and maintaining this resource. To achieve these goals, we have assembled a group of experts with pioneering contributions to transposon and genomics technologies in C. elegans. Our partner, Union Biometrica NV, European Scientific Operations, is a company specializing on platform technology development and will provide solutions essential for the automation and streamlining of the processes involved in generating libraries of mutant strains. The final product of our focused project-a comprehensive collection of transposon-tagged alleles-together with the acquisition of efficient transposon-based tools for mutagenesis and transgenesis in C. elegans, should be of great value to the European and international scientific community. Our efforts will strengthen European research in the strategic area of functional genomics and yield a wealth of information on gene function, immediately relevant to pharmaceutical R&D. Champ scientifique natural sciencesbiological sciencesneurobiologysocial sciencessociologyindustrial relationsautomationnatural sciencesbiological sciencescell biologymedical and health sciencesbasic medicinepathologynatural sciencesbiological sciencesgeneticsgenomes Mots‑clés Cacnorhabditis elegans Functional Genomics Gene Knock Gene Knock-Out Heterologous Transposition Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Appel à propositions FP6-2002-LIFESCIHEALTH Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS Contribution de l’UE Aucune donnée Adresse Vassilika Vouton 1527 HERAKLION Grèce Voir sur la carte Coût total Aucune donnée Participants (5) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE France Contribution de l’UE Aucune donnée Adresse 101 Rue de Tolbiac PARIS Voir sur la carte Coût total Aucune donnée CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France Contribution de l’UE Aucune donnée Adresse Rue Michel Ange 3 287 PARIS Voir sur la carte Coût total Aucune donnée MAIA SCIENTIFIC Belgique Contribution de l’UE Aucune donnée Adresse Cipalstraat, 3 GEEL Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITY OF BRISTOL Royaume-Uni Contribution de l’UE Aucune donnée Adresse Senate House, Tyndall Avenue BRISTOL Voir sur la carte Coût total Aucune donnée UNIVERSITE CLAUDE BERNARD LYON 1 France Contribution de l’UE Aucune donnée Adresse Bld du 11 novembre 1918, 43 VILLEURBANNE Voir sur la carte Coût total Aucune donnée