Obiettivo DNA double-strand breaks (DSBs) are highly cytotoxic and mutagenic. In eukaryotes, two proteins, Mre11 and Rad50 play a major role in DSBs recombination/repair. In particular, these proteins are involved in the maturation of DSBs during homologous recombin ation which requires 3' overhangs for recombinases. However, their in vitro activities cannot explain this maturation mechanism suggesting the existence of additional partners. In thermophilic archaea homologs of Mre11 and Rad50 are organised in an operon-like structure, together with two genes encoding a nuclease and a helicase. The nuclease (NurA) exhibits 5´-3´ exonuclease activity, indicating that it could be essential for the production of the 3´overhangs (Constantinesco et al., 2002). I´m interested i n the design of an in vitro system for DSBs maturation by the Rad50-Mre11 pathway. In the proposed study I will analyse the interactions between the four proteins Mre11, Rad50, NurA and HerA and investigate the activities of the proteins as well as of the potential complexes. Subsequently I will identify additional proteins binding to the complexes using crude protein extracts from Sulfolobus and specific antibodies. In addition, I will initiate the study of in vivo functions using recently developed geneti c tools for Sulfolobus. Together this should lead to a comprehensive model of the initiation step of DNA recombination/repair in archaea. Campo scientifico natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsnatural sciencesbiological sciencesgeneticsgenomes Parole chiave DNA processing DNA repair homologous recombination hyperthermophilic archaea molecular machine Programma(i) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Argomento(i) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Invito a presentare proposte FP6-2002-MOBILITY-5 Vedi altri progetti per questo bando Meccanismo di finanziamento IIF - Marie Curie actions-Incoming International Fellowships Coordinatore UNIVERSITE PARIS-SUD Contributo UE Nessun dato Indirizzo 15 rue Georges Clémenceau ORSAY Francia Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato