Biosynthesis of Plant Cell Wall Polysaccharides ¿ Glyco-metabolons and Host-pathogen Interaction

Projektinformationen

GLYTRANS

ID Finanzhilfevereinbarung: 21313

Startdatum 1 Februar 2006

Enddatum 31 Januar 2008

Finanziert unter

• Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006

Gesamtkosten € 0,00

EU-Beitrag € 24 948,00

Koordiniert durch

KOBENHAVNS UNIVERSITET
Denmark

Ziel

"A cell wall surrounds every cell in a plant. It supports and determines the plant¿s physical form and provides a barrier against invading pathogens. The wall polysaccharides are synthesized by glycosyltransferases (GTs) located in the Golgi vesicles. Char acteristic signature structures for Golgi targeting have not been defined and this raises a question as to how the targeting of the GTs occurs. Many Golgi enzymes are known to be targeted by interactions with other proteins. We reason that this could be pa rticularly true for enzymes involved in biosynthesis of complex polysaccharides, which are likely to be synthesized by protein complexes. The objective is to identify the mechanism for Golgi targeting of the GTs from the perspective of protein-protein inte raction. This targeting will be investigated using fluorescent fusion proteins and confocal laser scanning microscopy. Direct protein-protein interactions will be elucidated by comparing targeting in wild type background and in mutants lacking components o f the biosynthetic machinery as well as by fluorescence resonance energy transfer (FRET). Invading bacterial pathogens interact with the cell wall and in some instances form biofilm. Therefore, cell wall components, particularly the polysaccharide struc tures and oligosaccharides derived from these, may be important stimuli in this context. GT mutants will be analyzed for their susceptibility to bacterial attachment, biofilm formation, and subsequent disease development. Because microbial biofilm is a con tinuing problem in both medical and industrial settings, the results have potential for technological applications, such as for designing coating materials for medical devices and in the food industry. The specific scientific goals are: - To identify novel Golgi proteins interacting with known GTs. - To investigate protein-protein interactions in vivo in the Golgi apparatus. - To evaluate plant polysaccharides as substrates or inducers of microbial biofilm"

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins › proteomics
• natural sciences › biological sciences › microbiology › bacteriology
• natural sciences › biological sciences › biochemistry › biomolecules › carbohydrates
• natural sciences › physical sciences › optics › microscopy › confocal microscopy
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Schlüsselbegriffe

• Arabidopsis
• Golgi targeting protein-protein interaction biofilm
• Pseudomonas aeruginosa
• polysaccharides cell walls glycosyltransferases

Programm/Programme

• FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006

Thema/Themen

• MOBILITY-2.3 - Marie Curie Incoming International Fellowships (IIF)

Aufforderung zur Vorschlagseinreichung

FP6-2004-MOBILITY-7
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

IIF - Marie Curie actions-Incoming International Fellowships

Koordinator

KOBENHAVNS UNIVERSITET

• EU-Beitrag: Keine Daten
• Adresse: Noerregade 10 ; KOBENHAVN ; Dänemark
• Gesamtkosten: Keine Daten

Beteiligte (1)

THE ROYAL VETERINARY AND AGRICULTURAL UNIVERSITY

Dänemark

• EU-Beitrag: Keine Daten
• Adresse: 17 Bülowsvej ; FREDERIKSBERG
• Gesamtkosten: Keine Daten