Objective The human plasma proteome holds the promise of a revolution in disease diagnosis and therapy. One major breakthrough should come from the detection of multiprotein disease markers including isoforms. We propose to integrate a full proteomics analysis chain, from blood sample to the diagnosis information, combining bio-, nano, and information-related technologies. It includes an innovative patented lab-on-chip developed at CEA. The clinical application is early pancreatic cancer diagnosis.The project is based on a panel of 3 identified proteins, a protein isolation protocol, an optimised chromatographic-electrospray lab-on-chip, an Integrated Clinico-Proteomics Environment including a Proteomic Information Management System, a Clinical Information System, and modules for preprocessing, reconstruction, visualisation, protein identification, data mining and knowledge discovery. The clinical validation is applied to a cohort of 92 patients. Our targeted performance is to get at least the sensitivity of an orthogonal ELISA approach, to operate the analysis chain in less than 12 hours, and to demonstrate the interest of multiprotein marker.The main research outcomes will be an optimised chromatographic-electrospray lab-on-chip, a software environment supporting the integrated device, a proof-of-concept of their application to protein profiling for cancer diagnosis and an exploitation plan. The roadmap of this 36 months project is defined according to three main milestones: 1) at month 12, a first protein profile using a first version of the lab-on-chip on artificial samples is available, 2) at month 24, all the final versions of the sub-systems are ready for integration and validation, 3) at month 33, the validation on clinical samples is completed.The consortium partnership involves partners over 5 countries, combining basic and applied research (CEA, FORTH, SIB, WWU), 1 large company (ATOS) and 2 SMEs (BVN, GB), including clinicians and end-users (WWU, BVN). Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsengineering and technologyother engineering and technologiesmicrotechnologylab on a chipnatural sciencescomputer and information sciencesdata sciencedata miningsocial sciencespolitical sciencespolitical transitionsrevolutionsmedical and health sciencesclinical medicineoncologypancreatic cancer Keywords Nanotechnology Programme(s) FP6-IST - Information Society Technologies: thematic priority under the specific programme "Integrating and strengthening the European research area" (2002-2006). Topic(s) IST-2005-2.5.2 - Micro/nano based sub-systems Call for proposal Data not available Funding Scheme STREP - Specific Targeted Research Project Coordinator ATOS ORIGIN SOCIEDAD ANONIMA ESPANOLA EU contribution No data Address CALLE DE ALBARRACIN 25 28037 MADRID Spain See on map Total cost No data Participants (6) Sort alphabetically Sort by EU Contribution Expand all Collapse all COMMISSARIAT A L'ENERGIE ATOMIQUE France EU contribution No data Address 31-33 RUE DE LA FEDERATION 75752 PARIS CEDEX 15 See on map Total cost No data DIGILAB BIOVISION GMBH Germany EU contribution No data Address FEODOR-LYNEN-STRASSE 5 30625 HANNOVER See on map Total cost No data FOUNDATION FOR RESEARCH AND TECHNOLOGY - HELLAS Greece EU contribution No data Address VASSILIKA VOUTON 17671 IRAKLIO, CRETE See on map Total cost No data GENEVA BIOINFORMATICS (GENEBIO) SA Switzerland EU contribution No data Address AVENUE DE CHAMPEL 25 GENEVE See on map Total cost No data INSTITUT SUISSE DE BIOINFORMATIQUE Switzerland EU contribution No data Address CMU, MICHEL-SERVET 1 1211 GENEVE See on map Total cost No data WESTFAELISCHE WILHELMS-UNIVERSITAET MUENSTER Germany EU contribution No data Address SCHLOSSPLATZ 2 MUENSTER See on map Links Website Opens in new window Total cost No data