Objectif COCHISE is the first step of an activity aimed at the development of enabling micro-technologies to monitor physiological cellular interactions at the single cell level with a high throughput. It will be applied first to the immunological monitoring of anti-tumour vaccinations, singling out the rare effector cells (in the order of 10-3) that are actually active against tumour cells. The sensor that we are developing consists of an orderly matrix of about 4,000 living cells deposited in microwells created in a biocompatible substrate that also serves as a high-density circuit board.The microwells are monitored by an external microscope and have an embedded addressable impedance sensor. The key point is that each microwell can force contact between individual cells, and detect consequences of these contacts. The project integrates on the same platform several technologies such as electronic sensing, microfluidic interfaces for cell dispensing, control of osmotic balance of nutrients, management of evaporation, surface nano-modifications for management of fluid flows (e.g. hydrophilic and/or hydrophobic surface tend to drive or repel droplets) and avoidance or induction of surface cell adhesion. At the system level, cell delivery will leverage recent results that allow to delivery single cells in an effective way.An important side of the research is the definition of new therapeutic and diagnostic protocols for the immunotherapy of cancer. As a first step, we will apply our technology to the analysis of anti-tumour lytic effector cells, for a precise quantification of how many lytic events happen in the array, their locations and timings. A major advantage is that the cells are kept alive and can be retrieved individually for further analysis, such as gene expression profiling. Champ scientifique engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsnatural sciencesbiological sciencescell biologynatural sciencesphysical sciencesopticsmicroscopymedical and health sciencesbasic medicineimmunologyimmunotherapyengineering and technologyother engineering and technologiesmicrotechnology Mots‑clés Nanotechnology Programme(s) FP6-IST - Information Society Technologies: thematic priority under the specific programme "Integrating and strengthening the European research area" (2002-2006). Thème(s) IST-2005-2.5.2 - Micro/nano based sub-systems Appel à propositions Data not available Régime de financement STREP - Specific Targeted Research Project Coordinateur ALMA MATER STUDIORUM-UNIVERSITA DI BOLOGNA Contribution de l’UE Aucune donnée Adresse Via Zamboni 33 BOLOGNA Italie Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (7) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire AZIENDE CHIMICHE RIUNITE ANGELINI FRANCESCO - A.C.R.A.F. S.P.A. Italie Contribution de l’UE Aucune donnée Adresse VIALE AMELIA 70 00181 ROMA Voir sur la carte Coût total Aucune donnée COMMISSARIAT A L'ENERGIE ATOMIQUE France Contribution de l’UE Aucune donnée Adresse 31-33 RUE DE LA FEDERATION 75752 PARIS CEDEX 15 Voir sur la carte Coût total Aucune donnée FRAUNHOFER IAF Allemagne Contribution de l’UE Aucune donnée Adresse Tullastr. 72 79108 MÜNCHEN Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée INSTITUT DE PATHOLOGIE CELLULAIRE CHRISTIAN DE DUVE Belgique Contribution de l’UE Aucune donnée Adresse AVENUE HIPPOCRATE 75 1200 BRUXELLES Voir sur la carte Coût total Aucune donnée MICRONIT MICROFLUIDICS B.V. Pays-Bas Contribution de l’UE Aucune donnée Adresse HENGELOSESTRAAT 705 7521 PA ENSCHEDE Voir sur la carte Coût total Aucune donnée MINDSEEDS LABORATORIES - S.R.L. Italie Contribution de l’UE Aucune donnée Adresse VIALE ERCOLANI 3 40138 BOLOGNA Voir sur la carte Coût total Aucune donnée UNIVERSITA DEGLI STUDI DI FERRARA Italie Contribution de l’UE Aucune donnée Adresse SAVONAROLA 9 44100 FERRARA Voir sur la carte Coût total Aucune donnée
