Objectif The requirement to screen for known SNPs (single nucleotide polymorphisms) has become one of the key challenges to be addressed to enable the exploitation of the human genome sequence, where approximately 3 million SNPs are responsible for all of the variation within the human population. Several methods and technologies are currently available for detection of SNPs, but no single platform exists which can deliver 100% accuracy, in a low cost, versatile, and easy-to-use integrated system.The proposed research will focus on the development of integrated SNP detection platforms to include modules for DNA extraction and purification from biological samples, DNA amplification, DNA characterisation (including SNP detection), signal transduction, interpretation and data analysis. Integration of the modules on a single platform will include a transport mechanism based on electrowetting actuation (EWOD).Two alternatives SNP detection platforms will be developed based on optical and magnetic sensing respectively. The whole system will be packaged as a single automated functional system with a simplified GUI interface, based on specifications recommended by potential end-users within the consortium. This systems development will focus on delivering a product customised for low to medium throughput, low cost, point-of-care applications, with emphasis on providing very rapid and accurate results.Within the project, the systems will be benchmarked for screening of SNPs in the CFTR gene that are associated with cystic fibrosis. CF patient samples will be used to verify the accuracy and reproducibility of the system in a clinical diagnostics laboratory. Champ scientifique engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringcontrol systemsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsoptical sensorsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsnucleotides Mots‑clés Nanotechnology Programme(s) FP6-NMP - Nanotechnologies and nanosciences, knowledge-based multifunctional materials and new production processes and devices: thematic priority 3 under the 'Focusing and integrating community research' of the 'Integrating and strengthening the European Research Area' specific programme 2002-2006. Thème(s) NMP-2003-3.4.1.5-2 - Ethical, legal, social aspects of research in nanotechnology NMP-2004-IST-NMP-2 - Bio-sensors for Diagnosis and Healthcare Appel à propositions FP6-2004-IST-NMP-2 Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur UNIVERSITY COLLEGE CORK, NATIONAL UNIVERSITY OF IRELAND, CORK Contribution de l’UE Aucune donnée Adresse Lee Maltings, CORK Irlande Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (6) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire ASPER BIOTECH LTD Estonie Contribution de l’UE Aucune donnée Adresse Oru 3 TARTU Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée CHARLES UNIVERSITY PRAGUE Tchéquie Contribution de l’UE Aucune donnée Adresse Ovocny TRH 5 PRAGUE 1 Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée COMMISSARIAT A L'ENERGIE ATOMIQUE (CEA) France Contribution de l’UE Aucune donnée Adresse 31-33 rue de la Fédération PARIS Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA Portugal Contribution de l’UE Aucune donnée Adresse Campo Grande - Edificio C7 - 1 piso LISBOA Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée INSTITUTO DE ENGENHARIA DE SISTEMAS E COMPUTADORES PARA OS MICROSISTEMAS E AS NANOTECNOLOGIAS Portugal Contribution de l’UE Aucune donnée Adresse Rua ALVES REDOL 9 LISBOA Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée NATIONAL UNIVERSITY OF IRELAND, DUBLIN. UNIVERSITY COLLEGE DUBLIN Irlande Contribution de l’UE Aucune donnée Adresse Belfield DUBLIN Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée