Objectif ImmunoPrion proposal addresses the key words of FOOD-2004-T5.4.5.3: ???Investigating the fundamentals of TSE enabling the rational development of detection and control strategies ??? establish what defines a ???strain??? and what causes the ???species barrier effect???. TSE strains differ in incubation times, and neuro-anatomical PrPSc deposition. They may propagate through the food chain, and there is currently no EU certified diagnostic procedures for their identification. Our first objective is to provide a better definition of prion strains based on the structural characteristics of PrPSc. We will characterize in vitro synthesized PrP complexes and analyze the 3D native configuration of different prion strains in follicular dendritic cells derived from the gut, by high-resolution cryo electron tomography. Our second objective is to improve our capacity to predict prion transmission through the species barrier. One project is based on the use of a mouse model stably engrafted with human CD34+ stem cells, leading to a fully reconstituted lympho-reticular system. Backcrossing to transgenic mice will induce somatic expression of Hu-PrP in the CNS, to directly test the invasive properties of various prion strains. Another project will evaluate the role of immune surveillance against xenogeneic PrPSc and the influence of chronic intestinal inflammation. The third aim is to understand how complement mediates interactions between the different prion strains and the cells of the host immune system (phagocytes, DC). We will also look at markers of acquired immunity induced by the presence of PrPSc in infected hosts, in order to elaborate protocols for an early diagnosis of prion strains. The role of the SME is to create an interface between our consortium and industrial partners or institutions involved in food security, communication and education. All these objectives are organized around the idea that a rational food safety. Champ scientifique natural sciencesbiological sciencesneurobiologymedical and health sciencesbasic medicineimmunologymedical and health sciencesmedical biotechnologycells technologiesstem cellsengineering and technologyother engineering and technologiesfood technologyfood safetymedical and health scienceshealth sciencesnutrition Mots‑clés Neurodegenerative disease chronic infection immuno response Programme(s) FP6-FOOD - Food Quality and Safety: Thematic priority 5 under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) FOOD-2004-T5.4.5.3 - Investigating the fundamentals of TSE enabling the rational development of detection and control strategies Appel à propositions FP6-2004-FOOD-3-B Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE Contribution de l’UE Aucune donnée Adresse 101 rue de Tolbiac PARIS France Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (6) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Royaume-Uni Contribution de l’UE Aucune donnée Adresse University Offices, Wellington Square OXFORD Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITY OF ZURICH Suisse Contribution de l’UE Aucune donnée Adresse Raemistrasse 71 ZURICH Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée NEDERLANDS KANKER INSTITUUT Pays-Bas Contribution de l’UE Aucune donnée Adresse Plesmanlaan 121 AMSTERDAM Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée TECKNOWMETRIX SARL France Contribution de l’UE Aucune donnée Adresse PARC WORK CENTER RUE DU PLACYRE VOIRON Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France Contribution de l’UE Aucune donnée Adresse 3, rue Michel-Ange PARIS Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITÉ DE LIÈGE Belgique Contribution de l’UE Aucune donnée Adresse Place du 20 Aout, 9 LIÈGE Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée
