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Integrated polymer-based micro fluidic micro system for DNA extraction, amplification, and silicon-based detection

Objectif

The proposed research will develop a single device integrated micro system for nucleic acid extraction, mixing steps, purification, PCR amplification, biochemical reactions from biological samples e.g. blood, amplification, and DNA micro array detection in real time. The technologies that will be combined are silicon-based for the implementation of the detection and polymer-based for the micro fluidic part. The chip that will be developed in the project it will be ideally suited for being integrated in a portable low cost system that will perform the signal transduction, interpretation and data analysis.

The above described modules will be integrated within a single device where samples will be transported within and between modules via an enclosed micro fluidic channels system. The whole system will be interfaced by dedicated software that will be developed to make feasible and efficient the operation of the portable apparatus by general practitioners "packaged" as a portable automated functional system. The aim will be to deliver a product targeting low to medium throughput, low cost, point-of-care applications, with emphasis on providing very rapid and accurate results. The micro system that will be incorporated in the current research proposal will be modular, versatile, fully automated, requiring a minimum quantity of reagents and nucleic acid probes and reduced times for analysis.

Low density micro arrays will be printed accurately using a patented laser technique. Detection will be carried out by two alternative approaches, the photosensing and the capacitive, both implemented on the silicon device part. Both will be compared with an enhanced photographic method Micro heaters, temperature sensors, micro mixers will be also integrated into the micro system to enable good control of experiments and good reproducibility of hybridization results. The micro system that will be developed it will be validated for four different healthcare applications.

Champ scientifique

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Régime de financement

STREP - Specific Targeted Research Project

Coordinateur

Intracom S.A. Telecom Solutions
Contribution de l’UE
Aucune donnée
Adresse
New Road Peania - Markopoulo 19,7 Km
19002 PEANIA
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Dernière mise à jour: 10 Juillet 2012

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Permalink: https://cordis.europa.eu/project/id/027333/fr

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