Objectif Colorectal cancer is a leading cause of cancer in European non-smokers. Dietary changes might reduce the incidence up to 70%, according to epidemiological reports, but the dietary factor initiating them is not known. Researchers in the proposed outgoing organisation have recently found that rats given a diet with reduced thiamin (vitamin B1) for 4 months have small patches in their colons, ACF, that are recognised as precursors to colon cancer (Bruce et al, Cancer Lett. 2003). Furthermore, they have found with studies in tissue culture that the toxic effects of reduced thiamin are markedly worse when the cells are exposed to an oxidative stress (Shangari et al, Biochem. Soc. Trans. 2003).We want to use these preliminary findings as a base to find out whether the combination of reduced thiamin and an oxidative stress from cooked food increases the formation of ACF in the colons of rats at 4 months and whether it will result in colon cancer if extended for a longer period. This will be extended to other natural sources of oxidative stress such as red meat and sucrose. We also want to investigate biochemical measures from blood samples from these animals that can be used as biomarkers of thiamin deficiency and oxidative stress. The final stage would be to correlate biomarkers with the development of colon carcinogenesis in vivo. Our expectation is that these diet changes, reduced thiamin and cooked food, will result in colon cancer in the rat, and that the biochemical measures associated with the development of the tumours can be used to assess this risk in humans. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineoncologycolorectal cancermedical and health scienceshealth sciencesnutritionnatural scienceschemical sciencesorganic chemistryheterocyclic compoundsnatural scienceschemical sciencesorganic chemistryamines Mots‑clés ACF Antioxidant Biomarker Cancer Colon Haem Prevention Rat Sucrose Thiamin Vitamin B1 Vitamin_B1 Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.2 - Marie Curie Outgoing International Fellowships (OIF) Appel à propositions FP6-2002-MOBILITY-6 Voir d’autres projets de cet appel Régime de financement OIF - Marie Curie actions-Outgoing International Fellowships Coordinateur INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE (FRANCE) Contribution de l’UE Aucune donnée Adresse 147, Rue de l'Universite PARIS France Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire THE GOVERNING BODY OF TH UNIVERSITY OF TORONTO Canada Contribution de l’UE Aucune donnée Adresse TORONTO, ONTARIO Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée