Objectif The African trypanosome Trypanosoma brucei causes African sleeping sickness and the cattle disease nagana. The human and socio-economic impact of these diseases is considerable. The available drugs for humans are inadequate and difficult to administer. Parasites are very dependent on glycoconjugates for their survival and infectivity. The main goal of this project is to determine the function of genes involved in galactose-containing glycoconjugate biosynthesis and processing in T. brucei.The emerging T. bruce i genome will be systematically mined, and the candidate genes classified in different glycosyltransferase (GT) groups. Representative examples of these putative GTs will be experimentally examined by inducible RNAi knock-downs, by gene knockout, or by construction of tetracycline-inducible conditional null mutants. Mutant cells will be assessed for growth in vitro and for infectivity in vivo. Moreover, mutant cells will be bio-chemically phenotyped in both the blood stream and procyclic lifecycle forms of the parasite. In this way, we should be able to assign precise functions to genes and thus, identify sequence motifs/patterns that discriminate between different families of parasite enzymes. Champ scientifique medical and health sciencesclinical medicinepsychiatrysleep disordersagricultural sciencesanimal and dairy sciencedomestic animalsanimal husbandrynatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2002-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur UNIVERSITY OF DUNDEE Contribution de l’UE Aucune donnée Adresse Nethergate - DUNDEE Royaume-Uni Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée
