Cel Plants have evolved mechanisms to defend themselves. Defence mechanisms are often triggered after the direct or indirect interaction of a disease resistance (R) gene from the plant and an avirulence (Avr) gene from the pathogen. In the absence of the R gene or the Avr gene, disease ensues. Such "gene-for- gene" interactions are known for many different pathogens and plants. R proteins can be divided into five classes based on their structure. The focus of my work will be on the Receptor-like Protein (RLP) class, of which Cf-9 is the founding member.RLPs are membrane-anchored glycoproteins with extra-cytoplasmic Leucine Rich Repeat (LRRs, a protein-protein interaction motif), a transmembrane domain and a short cytoplasmic tail lacking any obvious signalling domain. The Cf-9 protein has 27 LRRs, which are divided in two blocks. Domain Cl comprises 23 LRRs, and the second, domain C3, has 4 LRRs. In between there is a putative "hinge" region, which does not have any LRRs, called domain C2. Sequence alignments of C2 between Cf-9 and Arabidopsis RLPs (AtRLPs) reveal conserved amino acids. Amino-acids surrounding the C2 region are also well conserved.The aim of the proposed project is to:(i) investigate the functional significance of amino acids conserved between Cf-9 and Arabidopsis RLPs, using transient assays of mutated Cf-9 alleles in Nicotiana benthamiana and(ii) to make chimeras of Cf- proteins and Arabidopsis proteins, that can be transiently assessed in transient assays in turnip, to try establish a Cf-9/Avr9 response in Arabidopsis that can be used for genetic studies on Cf-9 signal transduction. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural scienceschemical sciencesorganic chemistryamines Program(-y) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Temat(-y) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Zaproszenie do składania wniosków FP6-2002-MOBILITY-5 Zobacz inne projekty w ramach tego zaproszenia System finansowania IIF - Marie Curie actions-Incoming International Fellowships Koordynator THE SAINSBURY LABORATORY Wkład UE Brak danych Adres Norwich Research Park, Colney NORWICH Zjednoczone Królestwo Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych