Objectif Model organisms are essential to study the genetic basis of human diseases. Transgenic mammalian models, especially genetic knock-out mice catalysed the progress. To continue the advancement, further sophisticated and refined models are crucially needed to study the genetic basis and manifestations of numerous human diseases. In order to enhance our genetic understanding of human diseases, this proposal is aimed to develop new tools for the generation of transgenic laboratory animal models both in mouse and rat for comparative analyses of functional genomics in different species. In the mouse stable embryonic stem cell lines enable gene-targeting technology. However, the lack of such cell lines in most mammalians has so far prevented targeted genetic modifications.This proposal is aimed to use new tools such as nuclear replacement and gene targeting in somatic cells as a technological platform for producing new transgenic models. The primary aim is to further explore and validate the nuclear replacement system for safety and reliability as a technological platform. Our goal is also to generate the first gene targeted rat models. Relevant issues, such as the role of mitochondrial inheritance, somatic cell gene targeting and standardisation of newly generated genotypes by cryo-preservation will be addressed as well. Champ scientifique medical and health sciencesmedical biotechnologygenetic engineeringmedical and health sciencesmedical biotechnologycells technologiesstem cells Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) LSH-2003-1.1.0-1 - Topics for Specific Targeted Research Project in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Appel à propositions FP6-2004-LIFESCIHEALTH-5 Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur AGRICULTURAL BIOTECHNOLOGY CENTER Contribution de l’UE Aucune donnée Adresse Szent Gyorgyi Albert utca 4. GODOLLO Hongrie Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (5) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire BIOTALENTUM LTD. Hongrie Contribution de l’UE Aucune donnée Adresse Aulich Lajos 26 GÖDÖLLO Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée HELMHOLTZ ZENTRUM MÜNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) Allemagne Contribution de l’UE Aucune donnée Adresse Ingolstaedter Landstrasse 1 NEUHERBERG Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITY OF COPENHAGEN Danemark Contribution de l’UE Aucune donnée Adresse Noerregade 10 COPENHAGEN K Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITY OF NOTTINGHAM Royaume-Uni Contribution de l’UE Aucune donnée Adresse University Park NOTTINGHAM Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITY OF VETERINARY MEDICINE, VIENNA Autriche Contribution de l’UE Aucune donnée Adresse Veterinaerplatz 1 VIENNA Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée