Objectif Despite increasing knowledge about stroke, treatment options remain unsatisfactory. Because the transfer of neuroprotective agents from the animal model to the patient has turned out to be problematic, there is tremendous interest in new ways of neuroprotection that might be feasible in humans also, such as the induction of endogenous cerebral protection. These mechanisms have been shown to be inducible by a sublethal toxic stimulus in vitro and in animal models ("preconditioning", "Ischemic tolerance"). In a recent study on stroke patients with antecedent transient ischemic attacks, the applicant found evidence for the existence of ischemic tolerance in the human brain.The proposed project will address the following questions:1) What factors elicited by preconditioning cause ischemic protection?2) Is there a specific tissue ¿signature¿ of preconditioned tissue?3) What distinguishes preconditioned tissue in a following ischemia from first ever ischemic brains?Magnetic Resonance Imaging (MRI) will be applied to a rat model of hypoxic preconditioning and focal ischemia, to assess parameters of cerebral perfusion and tissue integrity and their spatial and temporal relation to the development of tolerance. To identify a vascular component of preconditioning, MRI at 7 Tesla will include highly sophisticated arterial spin labelling techniques for perfusion-weighted imaging, developed at the host institution. Co-registered histological data will provide information on the extent and character of ischemia-induced neuro genesis.This comprehensive approach will allow addressing our principal hypothesis: induction of ischemic tolerance is facilitated by a concerted process of protective mechanisms on cellular and vascular level, rather than by independent single events. The transfer of expertise to the Berlin Neuroimaging Centre will provide new MRI technology and exciting options for co-operation, thereby contributing to European science competitiveness. Champ scientifique medical and health sciencesbasic medicineneurologystrokeengineering and technologymedical engineeringdiagnostic imagingmagnetic resonance imagingnatural sciencesbiological scienceshistology Mots‑clés Magnetic Resonance Imaging ischemic tolerance preconditioning stroke Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.2 - Marie Curie Outgoing International Fellowships (OIF) Appel à propositions FP6-2002-MOBILITY-6 Voir d’autres projets de cet appel Régime de financement OIF - Marie Curie actions-Outgoing International Fellowships Coordinateur UNIVERSITAET ZUERICH Contribution de l’UE Aucune donnée Adresse Raemistrasse 71 ZURICH Suisse Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (2) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CENTER FOR FUNCTIONAL MAGNETIC RESONANCE IMAGING,UNIVERSITY OF CALIFORNIA, SAN DIEGO États-Unis Contribution de l’UE Aucune donnée Adresse 9500 Gilman Drive/ Osler LN LA JOLLA, SAN DIEGO Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée NEUROLOGY CHARITE, UNIVERSITAETSMEDIZIN BERLIN Allemagne Contribution de l’UE Aucune donnée Adresse Schumannstr. 20/21 BERLIN Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée
