Objective
Marine picoeukaryotes, referred as organisms smaller than 3 micrometers in size, are widespread in marine environments and contribute significantly to global primary productivity.
Despite their ecological importance, picoeukaryotes have come to the attention only in the last few years, with the development of new cultivating approaches and molecular techniques.
However, culturing misses a lot of species due to their inability to grow in artificial media. Molecular techniques based on 18S-rRNA or 16S-rRNA gene sequence analysis reveal taxonomic diversity, distribution and abundance of marine microbiota, but provide no data about physiological features of certain phylogenetic groups and their particular contribution to the net activity of the community.
The proposed study is aimed at discovering the major contributors to the microbial food web via their highly expressed genes.
There are two primary components of this project:
(i) construction of normalized cDNA libraries from natural picoeukaryotic assemblages and creating expressed sequence tag (EST) databases, and
(ii) construction of a large-insert environmental DNA (metagenomic) library from the same environment as (i). EST data sets will allow an understanding of how members of the natural picoeukaryotic co mmunity experience their environment. Moreover, EST data will be used to design probes based on the genes managing most ecologically important metabolic pathways, such as marine carbon cycling.
Screening the metagenomic library using these probes will iden tify the genomic fragments from which these genes are expressed. Screening the same library by 18S rRNA gene probes and full sequencing of chosen fragments will allow phylogenetic identification of picoeukaryotes engaged in key biogeochemical processes and will provide novel sequence information from these ecologically important species.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences genetics DNA
- social sciences economics and business economics production economics productivity
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
COVENTRY
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.