Objective
We propose to crystallize the complex of the prokaryotic ribosome or its 50S subunit with the SecYEG translocon. Two SecYEG heterotrimers bind to the ribosome at the end of the nascent polypeptide tunnel to form a transmembrane channel which is part of a translocase complex that allows for secreted and membrane embedded molecules to be transcribed through or into the membrane bilayer respectively.
Building on previously established expression and complex formation protocols that allowed the analysis of this complex via electron microscopy we will proceed to crystallize the complex. We will rely on a multi-pronged approach involving ribosomes and SecYEG proteins from various organisms, which are able to form heterologous complexes, as well as engineered/truncated versions of the SecYEG proteins.
Our goal is to solve the crystal structure of this complex at atomic resolution to shed light on the exact mechanisms of one of the most basic and important biological processes. Questions we hope the structure will help us address are how the channel is opened and closed, how membrane proteins are embedded into the bilayer from the channel and how the membrane seal is maintained throughout the whole process.
The proposed project contributes to the development of x-ray crystallography of macromolecular assemblies and membrane proteins which are both regarded as future focal points of structural biology. As such it is valuable for European research and also provides excellent future perspectives for the researcher.
The techniques involved in the project complement the skill-set of the researcher well and will leave him with well-rounded crystallographic expertise focused on two of the most promising areas in his field of research. Furthermore, funding this project would al low the researcher, who is from an objective 1 less-favoured region, to return to Europe from his current position in the United States.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- engineering and technology materials engineering crystals
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences biological sciences molecular biology structural biology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
ZURICH
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.