Objective A major future challenge in biology and medicine is to study biomolecules in their normal context in cells and tissues - in situ - to determine their sub-cellular localization, how they interact with other molecules and participate in signalling and control of cellular function in health and disease. The main purpose of this project is to develop new analytical procedures for the study of nucleic acids and proteins in situ, and to illustrate how they can enable novel biological studies for improved therapeutic targeting using cancer as the primary application area. The methods that will be developed are based on "proximity ligation" and "padlock probing", which are the first technologies to meet this challenge and to offer the sensitivity and specificity required for detailed studies of single bio-molecules in situ. We will refine these technologies and develop a spectrum of reagents for the analysis of specific markers of particular interest in oncology. Furthermore, software and algorithms for automatic user-independent in situ image analysis of "digital" single molecule events will be developed. These methods, reagents and algorithms will then be used by scientists within the project to verify the value of such systems in biological studies. The assays will primarily be used in a research setting, but their utility in diagnostic settings will also be explored. A carefully selected interdisciplinary consortium of world class researchers and companies from several European countries are joining forces for the execution of this project. Four companies, of which three are SMEs, are taking leading roles in the project underlining the commercial relevance of the project. The results of the project will constitute an important foundation for products addressing significant market needs and thereby allowing the participating SMEs to build sustainable and successful businesses in the forefront of biotechnology. Fields of science engineering and technologyindustrial biotechnologybiomaterialsbioplasticspolylactic acidnatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineoncologysocial scienceseconomics and businesseconomicssustainable economy Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2005-1.1.0-3 - Topic for STREPs dedicated to SMEs in the area of Fundamental knowledge and basic tools for functional genomics in all organisms LSH-2005-1.2.5-4 - Innovative research in post-genomics, which has high potential for application Call for proposal FP6-2005-LIFESCIHEALTH-7 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator OLINK AB EU contribution No data Address Rudbeck laboratory, Dag Hammarskjölds väg 20 UPPSALA Sweden See on map Links Website Opens in new window Total cost No data Participants (7) Sort alphabetically Sort by EU Contribution Expand all Collapse all UPPSALA UNIVERSITY Sweden EU contribution No data Address Sankt Olofsgatan 10B UPPSALA See on map Links Website Opens in new window Total cost No data DAKO DENMARK A/S Denmark EU contribution No data Address Produktionsvej 42 GLOSTRUP See on map Links Website Opens in new window Total cost No data IMMUN SYSTEM I.M.S. AB Sweden EU contribution No data Address Dag Hammarskjölds väg 26 UPPSALA See on map Links Website Opens in new window Total cost No data VISIOPHARM A/S Denmark EU contribution No data Address Bøge Allé 5 HØRSHOLM See on map Links Website Opens in new window Total cost No data TECHNICAL RESEARCH CENTRE OF FINLAND Finland EU contribution No data Address Vuorimiehentie 3 ESPOO See on map Links Website Opens in new window Total cost No data THE UNIVERSITY OF EDINBURGH United Kingdom EU contribution No data Address Old College, South Bridge EDINBURGH See on map Links Website Opens in new window Total cost No data LEIDEN UNIVERSITY MEDICAL CENTER Netherlands EU contribution No data Address Albinusdreef 2 9600 LEIDEN See on map Links Website Opens in new window Total cost No data
