Functional connectivity of developing hippocampal networks: characterization of “circuit-hubs”

Projektinformationen

CIRCUIT-HUBS

ID Finanzhilfevereinbarung: 219611

Abgeschlossenes Projekt

Startdatum 1 März 2008

Enddatum 28 Februar 2010

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 162 509,69

EU-Beitrag € 162 509,69

Koordiniert durch

UNIVERSITE DE LA MEDITERRANEE D'AIX-MARSEILLE II
France

Ziel

Oscillatory activity synchronized over a large population of neurons is the signature of the developing brain and play a major role in the construction of neuronal circuits. The hypothesis at the basis of our research proposal is that the initiation of synchronous network events is dependent on precise connectivity patterns of the neuronal network. Models of complex systems and networks offer solid tools to characterize mathematically the dynamics of neural circuits and predict the existence of networks “hubs”, i.e. neurons with spread and much larger number of connections, which strongly influence the state of the network. The aim of the present project is to identify specific types of neurons driving the oscillatory activity in the hippocampus during development. In order to find network “hubs”, the activity in neuronal circuits must be first measured with a high spatiotemporal resolution. Fast multibeam two-photon calcium imaging represents the most accurate experimental system to measure the activity of large populations of neurons with single-cell resolution and will be used for the experiments. Online analysis of the network temporal dynamics will identify candidate hubs. Patch clamp recordings from these neurons while imaging will further characterize the relationship between the state of the neuron and network dynamics. Morphological reconstruction and neurochemical characterization of recorded hubs will be performed post hoc. Offline accurate mathematical analysis will reconstruct and compare the temporal dynamics of the network in control conditions and during hub stimulation. My previous studies on neuronal circuits using multi-electrode arrays and my background in Physics give me the necessary experimental and theoretical background to carry on this study and we have already obtained very encouraging preliminary results. This novel approach will be of interest to a wide audience of researchers spanning from experimental neurobiology to network theory.

Wissenschaftliches Gebiet

• natural sciences › mathematics › pure mathematics › mathematical analysis

Schlüsselbegriffe

• Development
• GABA
• Hubs
• Interneurons
• Neuronal Networks
• Oscillations
• Two-photon imaging

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2007-2-1-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

UNIVERSITE DE LA MEDITERRANEE D'AIX-MARSEILLE II

Kontakt Verwaltung

Céline Damon (Ms.)

• EU-Beitrag: € 162 509,69
• Adresse: Boulevard Charles Livon, Jardin du Pharo 58 ; 13284 MARSEILLE ; Frankreich
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten