Obiettivo The Central Nervous System (CNS) is divided in different compartment structures: Forebrain, Midbrain and Hindbrain. The hindbrain in turn is subdivided in rhombomeres: compartmental structures separated by boundaries. It has been described that another CNS compartment borders are organizing centres, but it’s unknown if the hindbrain boundaries have this role and which are the molecular mechanisms that specify this territory, to be distinguished from the remaining hindbrain cells. Nevertheless, there are cues that suggest that hindbrain boundaries act like organizing centres: Activation of signalling pathways and stereotypic organization of neuronal groups. Our questions are: (i) What are the mechanisms that induce boundary cells at the segment interfaces? (ii) What is the behaviour and fate of boundary cells? And, (iii) What is the role of boundaries in the zebrafish hindbrain? To answer them we’ll map the regulatory region of rfng, a hindbrain boundary marker, in order to know the region that drives its expression only in boundaries. Through this approach we follow two objectives: (1) To know which genes regulate its expression, and (2) to obtain transgenic lines, which express reporters in boundaries. These tools will help us, through the use of time-lapse microscopy and hystochemistry to address the boundary cell fate, proliferation, and gene expression. Both approaches will help us to understand the regulatory complex that drives the boundary specification and differentiation and also to know if: the rhombomere boundaries act like organizing centres. Campo scientifico natural sciencesbiological sciencesneurobiologynatural sciencesphysical sciencesopticsmicroscopy Parole chiave Animal embryology Biological sciences Central Nervous System Neurobiology compartment boundary rhombomere zebrafish Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Invito a presentare proposte FP7-PEOPLE-2007-2-1-IEF Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IEF - Intra-European Fellowships (IEF) Coordinatore MEDICAL RESEARCH COUNCIL Contributo UE € 178 307,05 Indirizzo 20 Park Crescent W1B 1AL LONDON Regno Unito Mostra sulla mappa Tipo di attività Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Contatto amministrativo David Wilkinson (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
