Objective
Coeliac disease is an inflammatory disease of the upper small intestine that affects as much as 1% of the European population and results from gluten ingestion in genetically susceptible individuals. The only treatment for CD is a strict gluten-free diet, with the longer the individual fails to adhere to this diet, the greater the chance of developing malnutrition and other complications. Thus, the existence of reliable gluten free food is crucial to the well-being of the population. Current assays available for detection of gluten in food suffer from many drawbacks. Firstly, there appears increasing knowledge as to the toxicity of particular gluten proteins. Secondly, there is no existing protocol that has been demonstrated to quantitatively extract gluten from both raw and processed foodstuffs and assays based on the use of antibodies are incompatible with the reducing reagents. Fourthly, assays typically used for gluten detection exploit indirect sandwich assays, typically requiring 6-8 hours to complete. Here we propose to address each of the challenges to develop reagentless biosensors for in-situ detection of gluten in foodstuffs by using aptamers. Aptamers are getting more and more attention in biosensor area especially because of their superior properties over antibodies. Aptamers will be selected against these identified gluten toxic sequences in buffer conditions akin to that required for gluten extraction and are thus unaffected by the reducing agents present (contrary to the case of antibodies). Furthermore, aptamers lend themselves to highly flexible assay formats not attainable using antibodies. In the work proposed here, we intend to take special extraction protocol and selected aptamers and exploit them for use in various reagentless aptasensor formats (e.g. fluorescent-electrochemical displacement, molecular aptamer beacons) that will allow the rapid, inexpensive, accurate and facile detection of gluten in foodstuffs.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors biosensors
- engineering and technology other engineering and technologies food technology
- medical and health sciences health sciences inflammatory diseases
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences health sciences nutrition
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
43003 TARRAGONA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.