Objectif Unrepaired DNA breaks can lead to genomic instability or cell death. They occur frequently during normal cellular metabolism and are caused, for example, by the collapse or stalling of the replication fork in response to DNA damage. Proper DNA-end processing and handling are essential for the survival of the cell and prevention of carcinogenesis. Cells possess robust mechanisms to repair DNA breaks. One such DNA repair mechanism is homologous recombination where the sister chromatid is used as a template for the faithful repair of the DNA break. In Bacteria, this pathway is initiated when a DNA end is processed to a 3-ssDNA overhang terminated at a recombination hotspot (Chi) sequence. This is a substrate for formation of a RecA nucleoprotein filament that catalyses strand exchange to promote repair. Recent data implicate the AddAB helicase-nuclease and the SMC (Structural Maintenance of Chromosomes) complex in the DNA break processing mechanism of the model organism Bacillus subtilis. Interaction between these machines provides a molecular link between DNA dynamics and the initiation of DNA break processing that may co-ordinate replication fork collapse and DNA repair. Single-molecule manipulation and imaging techniques offer huge potential to investigate DNA break repair reactions in completely new ways, providing information that is inaccessible to conventional ensemble experiments. The aim of this project is two-fold: firstly, to develop novel biophysical instruments for fast Atomic Force Microscopy imaging in liquid and a combined Optical and Magnetic Tweezers setup; and secondly, to monitor and characterize the real-time dynamics of these DNA-repair processes using these new and complementary biophysical approaches. Single-molecule investigation will be supported by statistical analysis of the data and conventional bulk biochemical techniques. Champ scientifique natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsmicroscopynatural sciencesbiological sciencesgeneticschromosomes Mots‑clés AddAB enzyme Atomic Force Microscopy DNA repair Magnetic and Optical Tweezers SMC complex Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-SG-PE3 - ERC Starting Grant - Condensed matter physics Appel à propositions ERC-2007-StG Voir d’autres projets de cet appel Régime de financement ERC-SG - ERC Starting Grant Institution d’accueil AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Contribution de l’UE € 586 020,14 Adresse CALLE SERRANO 117 28006 Madrid Espagne Voir sur la carte Région Comunidad de Madrid Comunidad de Madrid Madrid Type d’activité Research Organisations Contact administratif Alberto Sereno Alvarez (Mr.) Chercheur principal Fernando Moreno Herrero (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Espagne Contribution de l’UE € 586 020,14 Adresse CALLE SERRANO 117 28006 Madrid Voir sur la carte Région Comunidad de Madrid Comunidad de Madrid Madrid Type d’activité Research Organisations Contact administratif Alberto Sereno Alvarez (Mr.) Chercheur principal Fernando Moreno Herrero (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée UNIVERSITY OF BRISTOL Royaume-Uni Contribution de l’UE € 582 408,00 Adresse BEACON HOUSE QUEENS ROAD BS8 1QU Bristol Voir sur la carte Région South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Bristol, City of Type d’activité Higher or Secondary Education Establishments Contact administratif Johanna Rule (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée FUNDACIO INSTITUT CATALA DE NANOCIENCIA I NANOTECNOLOGIA Participation terminée Espagne Contribution de l’UE € 455 801,86 Adresse CAMPUS DE LA UAB EDIFICI Q ICN2 08193 Cerdanyola Del Valles Voir sur la carte Région Este Cataluña Barcelona Type d’activité Research Organisations Contact administratif Carlos Manuel Abad Ruiz (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée