Obiettivo My research project is based on one principal axis: how extracellular matrix proteins participate to the functional organisation of the synapse. The goal of my research project is to study ColQ induced regulation of acetylcholine receptor (AChR) expression process. Recently, during a collaborative project between Jean Cartaud and Claire Legay groups, we have shown that the C-terminal part of ColQ interacts with MuSK (muscle-specific tyrosine kinase receptor) ectodomain. This association is not only responsible for anchoring AChE in the synaptic basal lamina but could also play an important role in regulating AChR expression. Other works have shown that ColQ interacts with another partner, the perlecan, an extracellular matrix heparan sulfate proteoglycan (HSPG) interacting with the Dystroglycan (DG). Based on these evidence, we hypothesized that ColQ could regulate the number of AChR via MuSK and/or perlecan/dystroglycan pathways. These signalling pathways would then be used as pivot adapting the number of AChE molecules to the number of AChR. Preliminary results from the laboratory confirm this hypothesis and suggest that ColQ regulates AChR and synaptic proteins expression and also suggest that ColQ may act via the two signalling pathways MuSK and perlecan/dystroglycan. Using various complementary approaches of biochemistry, cellular and molecular biology both in vitro and in vivo in mice, I will identify the signalling pathways induced by ColQ, in particular those involving ColQ/perlecan/dystroglycan and ColQ/MuSK. This study will allow me to better understand the mechanisms by which ColQ controls the postsynaptic domain differentiation at the NMJ. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesmolecular biology Parole chiave ColQ Neurobiology Neuromuscular junction motoneuron axon guidance signal signalling pathways Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) PEOPLE-2007-2-2.ERG - Marie Curie Action: "European Reintegration Grants" Invito a presentare proposte FP7-PEOPLE-2007-2-2-ERG Vedi altri progetti per questo bando Meccanismo di finanziamento MC-ERG - European Re-integration Grants (ERG) Coordinatore INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Contributo UE € 30 000,00 Indirizzo RUE DE TOLBIAC 101 75654 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Contatto amministrativo Claire Legay (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato