Objective
Quantum Dots (QDs) are a relative new semiconductor nanoparticles made from Cd/Se or Cd/Te with a shell of ZnS that have very excellent spectroscopic properties: broad absorption spectra, low photobleaching levels, narrow and symmetric emission bands, high quantum yields and large stoke shifts, which make them very attractive for fluorescence applications and, principally, for research studies in the biomedical field. The use of nanoparticles in vitro and in vivo has come in parallel with the development of water soluble QDs. However, together with the improvement in the solubility properties, there is a nascent necessity of developing efficient chemoselective methods of bioconjugation. This purpose is where is addressed this proposal to. We are going to try to use chemoselective ligations to attach peptides and proteins to QDs. In principle, we are interested in oxime, hydrazone reactions and [3+2] azide-alkyne cycloadditions, because it´s known that are very selective and happen with efficient rates at low µM concentrations (~10 µM) forming thermodynamically and kinetically stable products. Moreover, these ligation reactions have been used with success in peptide-peptide and peptide-dye conjugations. Once we have developed the conjugation methodology, we have in mind to apply it to the study of protein-nucleic acids and protein-protein interactions through the well-known FRET technique (Fluorescence Resonance Energy Transfer). We think that attaching a low number of proteins per quantum dot (around 5-10 protein molecules/QD) we can get high amplifications in the FRET signal, which will allow us to measure thermodynamic parameters like binding constants. To evaluate the method, we´ll study the system formed by GCN4 (a natural Transcription Factor) and its CRE recognition site (5´-…ATGACGTCAT…-3´), principally because there is a high literature data that help us to validate the approach.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics spectroscopy absorption spectroscopy
- natural sciences physical sciences electromagnetism and electronics semiconductivity
- engineering and technology nanotechnology nano-materials
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-1-IOF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
15782 SANTIAGO DE COMPOSTELA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.