Objective Although the definition of latency under a clinical point of view seems clear, the bacterial biology behind that clinical situation remains poorly understood. While dormant, the tubercle bacilli are considered to be under non-replicating (NR) stage. In such a condition, bacilli are not only difficult to be detected but also refractory to the standard treatments avoiding their clearance from the infected tissues. The proposal has been built with the intend of providing tools to understand the bacterial mechanisms that leads to metabolic stage of M. tuberculosis during dormancy as the basis of sorting out the detection and treatment of latent infection. Several models of analysis have been developed trying to characterize dormant tubercle bacilli. Those models are ranging from in vitro conditions, such as hypoxia or starvation, to in vivo analysis, such as the animal model. We propose not only to study a complete range of those previously tested conditions, but also checking some other putative newly described, such as the recently described adipocytes ex vivo model, the new developments of the classical model of hypoxia, and the use of guinea-pigs as more adequate animal model of latent infection. Moreover, a set of drug combinations will be applied to determine their capability of clearance of the bacterial load. Due to its central role in the bacterial metabolism and growth we will analyze the non-replicating stage of the M. tuberculosis bacilli by determining the pre-rRNA synthesis. Finally, the cellular and tissue distribution of dormant bacilli will be also tested during in vivo latent infection both in the animal model and in clinical human samples. Research on the metabolic conditions of the dormant bacilli inside hosts, will provide important and invaluable insight into the biology of M. tuberculosis. That knowledge may lead to the development of novel strategies targeted at the control of the latent infection. Fields of science natural sciencesbiological sciencesmicrobiologybacteriologymedical and health sciencesclinical medicinepneumologytuberculosis Keywords Dormancy models Latent Tuberculosis Treatment of latent TB Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH-2007-2.3.2-2 - Highly innovative approaches for research into host-pathogen interaction in tuberculosis HEALTH-2007-2.3.2-3 - Development of fast tests for the diagnosis of Multi-Drug-Resistant strains of HIV, malaria and tuberculosis and of latent tuberculosis infection (LTBI) Call for proposal FP7-HEALTH-2007-A See other projects for this call Funding Scheme CP-FP - Small or medium-scale focused research project Coordinator UNIVERSIDAD AUTONOMA DE MADRID EU contribution € 483 440,00 Address CALLE EINSTEIN 3 CIUDAD UNIV CANTOBLANCO RECTORADO 28049 Madrid Spain See on map Region Comunidad de Madrid Comunidad de Madrid Madrid Activity type Higher or Secondary Education Establishments Administrative Contact Ana Amigo (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (6) Sort alphabetically Sort by EU Contribution Expand all Collapse all ISTITUTO SUPERIORE DI SANITA Italy EU contribution € 468 299,50 Address Viale Regina Elena 299 00161 Roma See on map Region Centro (IT) Lazio Roma Activity type Research Organisations Administrative Contact Rosa Maria Martoccia (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data INSTITUT DE INVESTIGACIO EN CIENCIES DE LA SALUT GERMANS TRIAS I PUJOL Spain EU contribution € 310 160,00 Address CTRA DE CANYET S/N 08916 Badalona Barcelona See on map Region Este Cataluña Barcelona Activity type Research Organisations Administrative Contact Cristina Vilaplana Massaguer (Dr.) Links Contact the organisation Opens in new window Total cost No data ST GEORGE'S HOSPITAL MEDICAL SCHOOL United Kingdom EU contribution € 389 384,00 Address CRANMER TERRACE SW17 0RE London See on map Region London Inner London — West Wandsworth Activity type Higher or Secondary Education Establishments Administrative Contact Susan Mcpheat (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data INSTITUTO POLITECNICO NACIONAL Mexico EU contribution € 304 320,00 Address MIGUEL OTHON DE MENDIZABAL SN, COL. LA ESCALERA 07320 MEXICO DF See on map Activity type Higher or Secondary Education Establishments Administrative Contact Luis Antonio Jimenez-Zamudio (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data CORPORACION CORPOGEN Colombia EU contribution € 374 400,00 Address Carrera 5 N° 66A-34 N/A BOGOTA See on map Activity type Research Organisations Administrative Contact Luis Fernando Diaz (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS France EU contribution € 386 000,00 Address RUE MICHEL ANGE 3 75794 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Armelle Barelli (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
