Objective
The nucleus contains distinct sub-compartments, the composition and localisation of which change during normal physiological processes, or in response to stress or disease. The molecular stimuli that provoke these changes, and their effects on gene expression are not well characterised. This proposal aims to further define the mechanisms that underlie nuclear organisation, which is fundamentally important to most eukaryotic cell-types. Moreover, as there are many diseases linked with distinct changes in nuclear architecture, a better understanding of these mechanisms will aid in the development of new treatments, and diagnosis/screening procedures. Both these outcomes will reinforce the scientific excellence of Europe. My work will focus on the dynamic behaviour of Cajal bodies (CBs), which are conserved sub-nuclear domains involved in ribonucleoprotein-complex assembly. Recently, it was discovered that upon UV-C irradiation of cells, many CB proteins redistribute into “microfoci”. One of these proteins, coilin, displayed an increase in association with a novel binding partner after UV-C irradiation, Proteasome Activator subunit gamma (PA28g). Several lines of evidence suggest that the UV-C-induced redistribution of CBs occurs via a novel pathway that promotes the formation of PA28g/coilin complexes. I will extend these observations and determine the molecular mechanisms underlying the transduction of stress signals to the nucleus, by using biochemical and proteomic approaches (including a cutting-edge mass spectrometry technique; SILAC-MS) to identify novel proteins that show increased association with coilin/PA28g following UV-C irradiation. I will also determine the role of PA28g phosphorylation in the UV-C-induced redistribution of CBs by applying gene-silencing techniques and cell biological approaches. Finally, advanced fluorescence-microscopy methodologies will be combined with MS to determine if PA28g is functionally associated with the 20S proteasome.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-2-IIF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DD1 4HN Dundee
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.